DCS-Artículos

URI permanente para esta colecciónhttps://hdl.handle.net/10953/114

Examinar

Mostrando 1 - 20 de 37

A soft 3D polyacrylate hydrogel recapitulates the cartilage niche and allows growth-factor free tissue engineering of human articular cartilage
(Elsevier, 2019) Jiménez-González, Gema; Venkateswaran, Seshasailam; López-Ruiz, Elena; Perán, Macarena; Pernagallo, Salvatore; Díaz-Manchón, Juan José; Canadas, Raphaël; Antich, Cristina; Oliveira, Joaquim Miguel; Callanan, Anthony; Wallace, Robert; Reis, Rui L.; Montañez, Elvira; Carrillo-Delgado, Esmeralda; Bradley, Mark; Marchal, Juan Antonio
Cartilage degeneration or damage treatment is still a challenge, but, tissue engineering strategies, which combine cell therapy strategies, which combine cell therapy and scaffolds, and have emerged as a promising new approach. In this regard, polyurethanes and polyacrylates polymers have been shown to have clinical potential to treat osteochondral injuries. Here, we have used polymer microarrays technology to screen 380 different polyurethanes and polyacrylates polymers. The top polymers with potential to maintain chondrocyte viability were selected, with scale-up studies performed to evaluate their ability to support chondrocyte proliferation during long-term culture, while maintaining their characteristic phenotype. Among the selected polymers, poly (methylmethacrylate-co methacrylic acid), showed the highest level of chondrogenic potential and was used to create a 3D hydrogel. Ultrastructural morphology, microstructure and mechanical testing of this novel hydrogel revealed robust characteristics to support chondrocyte growth. Furthermore, in vitro and in vivo biological assays demonstrated that chondrocytes cultured on the hydrogel had the capacity to produce extracellular matrix similar to hyaline cartilage, as shown by increased expression of collagen type II, aggrecan and Sox9, and the reduced expression of the fibrotic marker’s collagen type I. In conclusion, hydrogels generated from poly (methylmethacrylate-co-methacrylic acid) created the appropriate niche for chondrocyte growth and phenotype maintenance and might be an optimal candidate for cartilage tissue-engineering applications.
Alteration of Postural Balance in Patients with Fibromyalgia Syndrome-A Systematic Review and Meta-Analysis
(MDPI, 2021-01-15) Núñez-Fuentes, David; Obrero, Esteban; Zagalaz, Noelia; Ibáñez-Vera, Alfonso Javier; Achalandabaso-Ochoa, Alexander; López-Ruiz, María del Carmen; Rodríguez-Almagro, Daniel; Lomas, Rafael
Balance problems are one of the most frequent symptoms in patients with Fibromyalgia Syndrome (FMS). However, the extent and nature of this balance disorder are not known. The objective of this work was to determine the best evidence for the alteration of postural balance in patients with FMS and analyze differences with healthy controls. To meet this objective, a systematic review with meta-analysis was performed. A bibliographical search was carried out in PubMed Medline, Scopus, Web of Science, CINAHL and SciELO. Observational studies that assessed postural balance in patients with FMS compared to healthy subjects in baseline conditions, were selected. In a random-effect model, the pooled effect was calculated with the Standardized Mean Difference (SMD) and its 95% confidence interval (CI). Nineteen studies reporting data of 2347 participants (95% female) were included. FMS patients showed poor balance with a large effect on static (SMD = 1.578; 95% CI = 1.164, 1.992), dynamic (SMD = 0.946; 95% CI = 0.598, 1.294), functional balance (SMD = 1.138; 95% CI = 0.689, 1.588) and on balance confidence (SMD = 1.194; 95% CI = 0.914, 1.473). Analysis of the Sensory Organization Test showed large alteration of vestibular (SMD = 1.631; 95% CI = 0.467, 2.795) and visual scores (SMD = 1.317; 95% CI = 0.153, 2.481) compared to healthy controls. Patients with FMS showed worse scores for different measures of postural balance compared to healthy controls. Concretely, FMS patients appear to have poor vestibular and visual scores with a possible somatosensory dependence.
Analysis of the microbiota of refrigerated chopped parsley after treatments with a coating containing enterocin AS-48 or by high-hydrostatic pressure
(Elsevier, 2017-09) Grande Burgos, María José; López Aguayo, María del Carmen; Pérez Pulido, Rubén; Gálvez, Antonio; Lucas, Rosario
El perejil puede estar implicado en enfermedades transmitidas por los alimentos , pero el perejil picado se utiliza como ingrediente o guarnición en múltiples platos. El objetivo del presente estudio fue determinar el efecto de dos tratamientos diferentes sobre la diversidad bacteriana del perejil: (i) recubrimiento con una solución de pectina-EDTA que contiene la bacteriocina circular enterocina AS-48, y (ii) tratamiento por alta presión hidrostática (HHP) a 600 MPa durante 8 min. El perejil control y tratado se almacenaron en bandejas a 5 °C durante 10 días. Ambos tratamientos redujeron los recuentos viables en 3,7 ciclos logarítmicos y retrasaron el crecimiento de los supervivientes durante el almacenamiento. La diversidad bacteriana del perejil picado se estudió mediante secuenciación de alto rendimiento (Illumina Miseq). La diversidad bacteriana de las muestras de control consiste principalmente en Proteobacteria (96,87%) pertenecientes a los géneros Pseudomonas (69,12%), Rheinheimera (8,56%) y Pantoea (6,91%), entre otros. Durante el almacenamiento, la abundancia relativa de Bacteroidetes (principalmente Flavobacterium y Sphingobacterium ) aumentó al 26,66%. La aplicación del recubrimiento de pectina-bacteriocina-EDTA redujo la abundancia relativa de Proteobacterias (63,75%) y aumentó la de Firmicutes (34,70%). Sin embargo, las abundancias relativas de ciertos grupos como Salmonella , Shigella y Acinetobacter aumentaron en las primeras épocas de almacenamiento. El almacenamiento tardío se caracterizó por un aumento en la abundancia relativa de Proteobacterias , principalmente Pseudomonas . Tras la aplicación del tratamiento HHP, la abundancia relativa de Proteobacteria se redujo (85,88%) mientras que Actinobacteria aumentó (8,01%). Durante el almacenamiento inicial de muestras tratadas con HHP,aumentó la abundancia relativa de Firmicutes . Las bacterias potencialmente patógenas ( Shigella ) sólo aumentaron en abundancia relativa al final del almacenamiento. Los resultados del presente estudio indican que los dos tratamientos tuvieron efectos diferentes sobre la diversidad bacteriana del perejil. El tratamiento HHP proporcionó un producto más seguro, ya que no se detectaron bacterias potencialmente patógenas hasta el final del período de almacenamiento.
Antimicrobial Activity and Biodiversity Study of a Homemade Vegetable Puree Treated with Antimicrobials from Paenibacillus dendritiformis
(2023-06-07) Mena, Laura; Grande, M.J.; Gálvez, A.
El Paenibacillus dendritiformis UJA2219 aislado de la zanahoria produce una actividad antibacteriana de amplio espectro. El objetivo del presente estudio fue determinar el impacto de los extractos de cultivos celulares parcialmente purificados de la cepa UJA2219 sobre la carga microbiana y la diversidad bacteriana de un puré de verduras casero. El puré se expuso a un cultivo de la cepa UJA2219 durante la noche o a extractos de caldo de cultivo parcialmente purificados mediante cromatografía de intercambio catiónico (CE) o cromatografía de fase reversa (RP) e incubados durante 7 días a temperaturas de 4 °C o 25 °C. Los mejores resultados se obtuvieron a 25 °C con el extracto de RP, disminuyendo los recuentos de las presuntas Enterobacteriaceae por debajo de los niveles detectables. La diversidad bacteriana del puré de control y tratado se estudió mediante secuenciación de extremos emparejados de Illumina, utilizando ADN extraído de las muestras de puré incubadas a 25 °C durante 24 h. Los controles y el puré inoculado con la cepa UJA2219 mostraron un perfil de diversidad bacteriana casi idéntico, con Pseudomonadota (principalmente Fam. Pseudomonadaceae -gen. Pseudomonas - y Enterobacteriace como los grupos más abundantes). Las mayores diferencias en la diversidad bacteriana se obtuvieron en el puré tratado con extracto de RP, mostrando una disminución en la abundancia relativa de Pseudomonadota (especialmente gen. Pseudomonas ) y un aumento de Bacillota (principalmente de los géneros Bacillus , Enterococcus y Lactococcus ). Los resultados del estudio sugieren que las preparaciones antimicrobianas de la cepa UJA2219 tienen potencial de aplicación en la biopreservación de alimentos.
Antimicrobial Resistance, Biocide Tolerance, and Bacterial Diversity of a Dressing Made from Coriander and Parsley after Application of Treatments Using High Hydrostatic Pressure Alone or in Combination with Moderate Heat
(MDPI, 2022-08-29) Rodríguez-López, Javier; Grande-Burgos, María José; Pérez-Pulido, Rubén; Iglesias-Valenzuela, Belén; Gálvez-del-Postigo, Antonio; Lucas-López, Rosario
The effects of high-hydrostatic pressure (HP) treatments (450 and 600 megapascals, MPa, for 5 min at temperatures of 22 C and 50 C) on the microbiota of a coriander and parsley dressing was studied via culture-dependent and culture-independent approaches. Samples were refrigerated for 20 days, with periodic counts of the culture media supplemented with, or without, antimicrobials. HPtreated samples showed significantly lower viable cell counts compared to untreated controls. Only the control samples yielded bacterial growth on media with antimicrobials (imipenem, cefotaxime, benzalkonium chloride), including mostly Pseudomonas and Lactobacillus. Bacillus and Paenibacillus were identified from pressurized samples. Few isolates showed higher tolerance to some of the biocides tested. Pseudomonads showed outstanding resistance to meropenem and ceftazidime. According to high-throughput sequencing analysis, the microbiota of the dressing control samples changes during storage, with a reduction in the relative abundance of Proteobacteria and an increase in Firmicutes. The composition of the residual microbiota detected during storage was highly dependent on the pressure applied, and not on the treatment temperature.
Associations between the impact of menopausal symptoms and fall-related self-efficacy
(Lippincott, 2023-04-01) Espírito-Santo, João; Marques de Loureiro, Nuno Eduardo; Brandão Loureiro, Vânia; Aibar-Almazán, Agustín; Hita-Contreras, Fidel
Objective: To analyze the association between the severity of menopausal symptoms and two important fall risk factors, namely balance confidence and fear of falling, among Portuguese and Spanish postmenopausal women aged ≥ 65 years. Methods: A cross-sectional, observational study was conducted on 363 women (66.21 ± 9.00 years) from several Portuguese and Spanish locations. The Menopause Rating Scale (MRS) was used to evaluate the severity of menopausal symptoms, while the Falls Efficacy Scale-International and Activities-specific Balance Confidence Scale-16 items were used to assess balance confidence and fear of falling, respectively. Anxiety and depression (evaluated by the Hospital Anxiety and Depression Scale), age, time since the onset of menopause, body mass index (BMI), history of falls, osteoporosis, smoking habit, physical activity level, and nocturia were considered as potential confounders. Independent associations were analyzed after adjusting for potential confounding variables. Student’s t test, bivariate correlations and multivariate linear regression analysis were performed. Results: A total of 363 women (66.21 ± 9.00 years) participated in the study, 192 from Portugal and 171 from Spain. Linear regression analysis indicates that more severe menopausal symptoms at a somato-vegetative level (beta coefficient (β) = -0.25; 95% confidence interval (95% CI):-2.09 to -0.81; p = <0.001), a higher BMI (β = -0.16; 95% CI:-1.22 to -0.22; p = 0.005), and osteoporosis (β = 0.14; 95% CI: 1.36 to 10.08; p = 0.010) were associated with lower balance confidence values. On the other hand, a higher score in the MRS somato-vegetative domain (β = 0.22; 95% CI: 0.27 to 0.79; p = <0.001), depression (β = 0.36; 95% CI: 0.59 to 1.08; p = <0.001), and years after the menopause onset (β = 0.15; 95% CI: 0.04 to 0.22; p = 0.006) were linked to increased fear of falling. Conclusions: The findings of our study show that, after taking into account possible confounders, increased severity of menopausal symptoms at a somatic level was associated with heightened fear of falling and diminished balance confidence.
Biofilms formed by microbiota recovered from fresh produce: Bacterial biodiversity, and inactivation by benzalkonium chloride and enterocin AS-48
(Elsevier, 2017-04-01) Grande Burgos, María José; Pérez Pulido, Rubén; Gálvez, Antonio; Lucas López, Rosario
Se permitió que la microbiota recuperada de productos frescos (lechuga romana, endivias y pepinos) formara biopelículas en cupones de acero inoxidable. Las biopelículas formadas se trataron con cloruro de benzalconio (BC) en tres concentraciones diferentes (0,01, 0,1 y 1,0 g/l), enterocina AS-48 (50 μg/ml) y combinaciones de BC y enterocina AS-48. El tratamiento único con bacteriocina no tuvo efecto sobre la viabilidad de las bacterias sésiles. Se requirió una alta concentración de BC (1,0 g/l) para lograr una reducción de 4,1 logs en los recuentos de células viables. La combinación de BC (1,0 g/l) y enterocina AS-48 redujo los recuentos de células viables por debajo de niveles detectables. El análisis de secuenciación de alto rendimiento reveló que las biopelículas formadas estaban compuestas principalmente por Proteobacterias de los géneros Pseudomonas y Stenotrophomonas. Los tratamientos con enterocina AS-48 y BC en concentraciones subinhibitorias solo indujeron cambios menores en la abundancia relativa de los diferentes grupos bacterianos asociados con las biopelículas. © 2016 Elsevier Ltd.
Brain, Heart and Kidney Correlate for the Control of Blood Pressure and Water Balance: Role of Angiotensinases.
(Karger, 2014-10) Prieto-Gómez, María Isabel; Villarejo, Ana Belén; Segarra, Ana Belén; Banegas, Inmaculada; Wangensteen, Rosemary; Martínez-Cañamero, Magdalena; de-Gasparo, Marc; Vives, Francisco; Ramírez-Sánchez, Manuel
The renin-angiotensin system (RAS) plays a major role in the control of blood pressure (BP) and water balance by coordinating brain, heart and kidney functions, connected with each other by hormonal and neural mechanisms through the autonomic nervous system (ANS). RAS function may be monitored by the study of the enzymes (angiotensinases) involved in the metabolism of its active peptides. In order to study the relationship between the brain-heart-kidney axis and the control of BP and water balance, we analyzed the correlation of angiotensinase activities, assayed as arylamidase activities, between hypothalamus, left ventricle, renal cortex and renal medulla, collected from Wistar-Kyoto and spontaneously hypertensive rats, treated or not treated with L-NAME [N(G)-nitro-L-arginine methyl ester]. This compound not only inhibits the formation of nitric oxide but also disrupts the normal function of the ANS activating the sympathetic nervous system (SNS) to increase BP. In addition, to assess the influence of the SNS, we studied the effect of its blockade by treatment of both strains with propranolol. The present results support the notion that RAS function of the brain-heart-kidney axis, as reflected by the activities of angiotensinases, is reciprocally connected by afferent and efferent mechanisms between these locations, presumably through the ANS. These results reveal new aspects of neuroendocrine regulation possibly involving the ANS.
Cellular extracts from post-mortem human cardiac tissue direct cardiomyogenic differentiation of human adipose tissue-derived stem cells
(Elsevier, 2013) Perán, Macarena; López-Ruiz, Elena; González-Herrera, Lucas; Bustamante, Milán; Valenzuela, Aurora; Marchal, Juan Antonio
Background aims. Human adipose tissue-derived stem cells (hASCs) can be easily (and inexpensively) expanded in culture, and their high plasticity allows their conversion to different cell types. We study the potential capacity of postmortem cardiac tissue to direct cardiac differentiation of hASCs in vitro. Methods. Cardiac tissue collected from autopsies was used to obtain cell extracts and conditioned medium, and both approaches were tested for cardiac induction. Results. Gene expression analyses proved that post-mortem human cardiac tissue maintains genetic integrity. hASCs exposed to the cell extracts or conditioned medium for 2 weeks achieved the appearance of myotube-like structures and were positive for cardiac markers such as sarcomeric a-actinin, cardiac troponin I and T and desmin as proved by immunofluorescence. In addition, differentiated cells showed increased expression of cardiomyocyte-related genes analyzed by reverse transcriptase polymerase chain reaction (GATA-4, myocyte-enhancer factor-2c, a-cardiac actin and cardiac troponin I). Conclusions. For the first time, post-mortem human cardiac tissue was used to induce hASC differentiation into myocardial-like cells. The methodology described here would serve as a useful model to obtain cardiomyocyte-like cells in vitro.
Changes in bacterial diversity of refrigerated mango pulp before and after treatment by high hydrostatic pressure
(Elsevier, 2017-05-01) Pérez Pulido, Rubén; Grande Burgos, María José; Gálvez, Antonio; Lucas, Rosario
El propósito del presente estudio fue determinar el efecto del tratamiento con alta presión hidrostática (APH) sobre la microbiota de la pulpa de mango. La pulpa de mango se contaminó artificialmente con su propia microbiota epífita para simular el peor de los casos de contaminación durante la preparación de la pulpa. Los controles y las muestras tratadas con HHP (600 MPa, 8 min) se almacenaron en frío durante 30 días. El tratamiento con HHP redujo significativamente (P <0,05) el recuento de células viables. El análisis de pirosecuenciación de la comunidad bacteriana reveló que las abundancias relativas encontradas en las muestras de control iniciales de Actinobacteria (45,63%), Firmicutes (42,55%), Proteobacteria (10,68%) y Bacteroidetes (1,0%) cambiaron durante el almacenamiento, con un fuerte aumento de Proteobacterias. El tratamiento con HHP también indujo un fuerte aumento de Proteobacterias, seguido de una recuperación tardía de Firmicutes y, en menor medida, de Actinobacterias. Lactobacillus fue la principal unidad taxonómica operativa (OTU) detectada tanto en los controles durante el almacenamiento temprano como en las muestras tratadas con HHP durante el almacenamiento tardío. Los resultados del presente estudio indican cómo las poblaciones bacterianas tanto de los controles como de las muestras de pulpa de mango tratadas con HHP sufren cambios complejos durante el almacenamiento en refrigeración. © 2016 Elsevier Ltd.
Changes in the bacterial diversity of human milk during late lactation period (Weeks 21 to 48)
(2020-08-27) Marín Gómez, Wendy; Grande Burgos, María José; Pérez Pulido, Rubén; Gálvez, Antonio; Lucas, Rosario
Se recolectó leche materna de una madre soltera durante un período de lactancia de 28 semanas. La diversidad bacteriana se estudió mediante análisis de secuenciación de amplicones de la región variable V3-V4 del gen 16S rRNA. Firmicutes y Proteobacteria fueron los principales filos detectados en las muestras de leche, seguidos de Actinobacteria y Bacteroidetes . La proporción de Firmicutes a Proteobacteria cambió considerablemente dependiendo de la semana de muestreo. Se detectó un total de 411 géneros o taxones superiores en el conjunto de muestras. El género Streptococcus se detectó durante el período de muestreo de 28 semanas, en abundancias relativas entre 2.0% y 68.8%, y fue el grupo más abundante en 14 de las muestras. Carnobacterium y Lactobacillus tuvieron abundancias relativas bajas. A nivel de género, la diversidad bacteriana cambió considerablemente en ciertas semanas dentro del período estudiado. Las semanas o períodos con menor abundancia relativa de Streptococcus presentaron composiciones bacterianas más diversas, incluyendo géneros pertenecientes a Proteobacteria que estaban poco representados en el resto de las muestras.
Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds.
(Wiley Online Libr, 2019-04-25) Glibota, N.; Grande, M.J.; Gálvez, A.; Ortega, E.
La contaminación por metales pesados puede actuar como una presión selectiva persistente que favorece la propagación de la resistencia a los antimicrobianos en entornos naturales. El objetivo de este estudio fue aislar e identificar bacterias tolerantes a metales en suelos de campos de olivos tratados rutinariamente con compuestos derivados del cobre y evaluar la tolerancia de las cepas bacterianas a otros metales y su resistencia a antibióticos clínicamente relevantes. Resultados: Se estudiaron 595 aislados bacterianos procedentes de 45 campos de cultivo de olivo. Se detectaron concentraciones mínimas inhibitorias (CMI) ≥ 16 mmol L -1 para cobre (57% de los aislados), zinc (37%) y plomo (62%), mientras que sólo el 3% presentó CMI ≥ 12 mmol L -1 para níquel. Se seleccionaron 96 cepas tolerantes a metales para su identificación y determinación de la resistencia a antibióticos. La mayoría de los aislados pertenecían a los géneros Pseudomonas (37%), Bacillus (23%) y Chryseobacterium (20%), mientras que el 6% se identificó como Variovorax, el 4% como Stenotrophomonas y el 2% como Serratia o Burkholderia. La mayor tolerancia al cobre se detectó entre Pseudomonas. Más del 75% de las cepas con alta tolerancia al cobre también fueron resistentes a la vancomicina, el 50% a la ampicilina y el 40% a la eritromicina o trimetoprima/sulfametoxazol. Conclusión: Las bacterias de los suelos del olivar son tolerantes a los metales, principalmente al cobre, pero también al zinc y al plomo, así como resistentes a antibióticos de importancia clínica, lo que podría suponer un problema en el ámbito clínico. © 2019 Sociedad de la Industria Química.
Corrective exercise-based therapy for adolescent idiopathic scoliosis: Systematic review and meta-analysis.
(SAGE, 2022) Gámiz-Bermúdez, Francisca; Obrero-Gaitán, Esteban; Zagalaz-Anula, Noelia; Lomas-Vega, Rafael
Abstract Objective: to analyse the efficacy of Corrective exercise-based therapy in the improvement of deformity and quality of life in adolescent idiopathic scoliosis. Data Sources: PubMed Medline, Scopus, Web of Science, Physiotherapy Evidence Database, CINAHL Complete and SciELO, until June 2021. Review methods: Randomized controlled trials was selected, including participants diagnosed with adolescent idiopathic scoliosis, in which the experimental group received Corrective exercise-based therapy. Two authors independently searched the scientific literature in the data sources, extracted the data and assessed the risk of bias. A pairwise meta-analysis using the random-effects model was performed. Results: Eight randomized controlled trials providing data from 279 adolescent idiopathic scoliosis patients were included. Seven randomized controlled trials including 236 patients showed moderate-quality evidence for a medium effect (SMD=-0.52, 95% CI -0.96 to -0.1), favouring corrective exercise-based therapy for spinal deformity reduction. Corrective exercise-based therapy was better than no intervention (SMD=-0.59, 95% CI -1.18 to -0.01) but similar to other intervention (SMD=-0.2, 95% CI -0.67 to 0.27), and a medium effect was found (SMD=-0.51, 95% CI -0.89 to -0.13) when corrective exercise-based therapy was used with other therapies. Four studies including 151 patients showed low-quality evidence of a large effect of Corrective exercise-based therapy on Scoliosis Research Society measurement (SRS-22) total score improvement (SMD=1.16, 95% CI 0.36 to 1.95). Conclusion: In mild and moderate adolescent idiopathic scoliosis patients, corrective exercise-based therapy could be used to reduce spinal deformity and to improve quality of life as isolated treatment or as coadjuvant treatment combined with other therapeutic resources.
Depression as a Risk Factor for Dementia and Alzheimer’s Disease
(MDPI, 2020-10-28) Cantón Habas, Vanesa; Rich-Ruiz, Manuel; Romero Saldaña, Manuel; Carrera-González, María del Pilar
Digital and Interactive Health Interventions Minimize the Physical and Psychological Impact of Breast Cancer, Increasing Women's Quality of Life: A Systematic Review and Meta-Analysis
(MDPI, 2022-08-26) Obrero Gaitán, Esteban; Cortés Pérez, Irene; Calet Fernández, Tania; García López, Héctor; López Ruiz, María del Carmen; Osuna Pérez, María Catalina
Digital and interactive health interventions (DIHIs), such as virtual-reality-based therapy (VRBT) and smartphone-app-based therapy (SABT), may be useful for reducing the impact of the signs and symptoms of breast cancer (BC) in women. The aim of this meta-analysis was to explore the effect of DIHIs on improving pain, anxiety, depression, quality of life (QoL), and upper extremity (UE) disability-related lymphedema in women with BC. Methods: We searched PubMed Medline, Web of Science, Scopus, CINAHL, Physiotherapy Evidence Database, and SciELO for the period ending February 2022. We included studies that assessed the effect of DIHIs on UE motor disability, pain, anxiety, depression, and QoL in women with BC. The effect size was calculated using Cohen's standardized mean difference (SMD) and its 95% confidence interval (95% CI). Results: Twenty studies providing data from 1613 women with BC were included. With respect to UE disability, DIHIs increased flexion (SMD, 1.92; 95%CI: -1.16, 2.68), abduction (SMD, 1.66; 95%CI: 0.91, 2.42), external rotation shoulder range of motion (SMD, 1.1; 95%CI: 0.36, 1.85), UE function (SMD, -0.72; 95%CI: -1.31, -0.13), and handgrip strength (SMD, 0.4; 95%CI: 0.21, 0.59). DIHIs reduced pain (SMD, -0.8; 95%CI: -1.31, -0.26), anxiety (SMD, -1.02; 95%CI: -1.71, -0.34), and depression (SMD, -1.57; 95%CI: -3.1, -0.08). Finally, DIHIs increased overall health (SMD, 0.6; 95%CI: 0.31, 0.89). Conclusions: Right at the end of therapy, DIHIs are effective at improving UE function, pain, anxiety, depression, and QoL in women with BC. VRBT has a greater effect than SABT for the assessed outcomes.
Does participation in therapeutic exercise programs after finishing oncology treatment still ensure an adequate health status for long-term breast cancer survivors? A ≥ 5 years follow-up study
(SPRINGER, 2023-05-18) Álvarez-Salvago, Francisco; Jiménez-García, José Daniel; Pujol-Fuentes, Clara; Atienzar-Aroca, Sandra; Molina-García, Cristina; Aibar-Almazán, Agustín
Purpose: The aims of this study were to evaluate whether the effects of two therapeutic exercise programs are sustained over time (≥ 5 years) in long-term breast cancer survivors (LTBCS). Second, to determine the influence of the current level of physical activity (PA) performed on cancer-related fatigue (CRF) that these patients may present ≥5 years later. Methods: A prospective observational study was conducted with a cohort of 80 LTBCS in Granada during 2018. Firstly, considering their participation in one of the programs, they were allocated into two groups: usual care and therapeutic exercise program, to assess CRF, pain and pressure pain sensitivity, muscle strength, functional capacity, and quality of life. Secondly, they were also classified into 3 groups according to current level of weekly PA performed: ≤ 3, 3.1-7.4, and ≥ 7.5 (MET-hour/week) respectively, to assess its impact over CRF. Results: Although the positive effects of the programs are not sustained over time, a trend toward significance can be observed for a greater reduction in overall CRF levels, lower intensity of pain in the affected arm and cervical region, and greater functional capacity and quality of life in the group that underwent therapeutic exercise. Additionally, 66.25% of LTBCS are inactive ≥ 5 years after completion of the program and furthermore, such inactivity is accompanied by higher CRF levels (P .013 to .046). Conclusion: The positive effects of therapeutic exercise programs are not maintained over time for LTBCS. Additionally, more than half of these women (66.25%) are inactive ≥ 5 years after completion of the program, this inactivity being accompanied by higher levels of CRF.
Effect of combined physical–cognitive training on the functional and cognitive capacity of older people with mild cognitive impairment: a randomized controlled trial
(BMC, 2024-07-08) Castellote-Caballero, Yolanda; Carcelén-Fraile, María del Carmen; Aibar-Almazán, Agustín; Afanador-Restrepo, Diego; González-Martín, Ana María
Background: The increase in population aging highlights the growing prevalence of mild cognitive impairment, prompting the adoption of interventions that combine physical exercise and cognitive training to improve health and cognitive performance in older adults. The aim of this study was to analyze the efficacy of a combined program on physical and cognitive health in older people with cognitive impairment. Methods: A 12-week randomized controlled clinical trial involving 95 participants (aged 72.12 ± 4.25 years), 47 individuals participated in a control group (CG) that only underwent cognitive stimulation, while 48 individuals were in an experimental group (EG) that participated in a combined program. Balance was measured using the Tinetti scale, upper body strength was assessed with the arm curl test, lower body strength was evaluated with the 30-s chair stand test, flexibility was tested using the back scratch test and chair sit-and-reach test, physical function was measured with the Timed Up and Go test, cognitive function was assessed using the Mini Mental State Examination, cognitive impairment was evaluated with the Montreal Cognitive Assessment, verbal fluency was tested with the Isaac test, and executive functions were assessed using the Trail Making Test. Results: The results of the study show significant improvements in both physical and cognitive aspects, such as balance, gait, upper and lower body strength, flexibility, physical function, cognitive function, cognitive impairment, verbal fluency, and executive functions in the group that carried out the intervention compared to the control group. Conclusion: A combined program for older individuals with mild cognitive impairment leads to enhancements in physical and cognitive health. These improvements underscore the importance of integrating physical exercise with cognitive training as an effective strategy for enhancing overall health and quality of life in older adults.
Effect of High Hydrostatic Pressure Processing on the Microbiological Quality and Bacterial Diversity of Sous-Vide-Cooked Cod
(MDPI, 2023-03-13) Pérez-Alcalá, Diego; Grande Burgos, María José; Rodríguez-López, Javier; Lucas-López, Rosario; Gálvez-del-Postigo, Antonio; Pérez-Pulido, Rubén
High hydrostatic pressure (HP) is a promising method to improve the microbiological quality of sous-vide foods. Monitoring the composition and behavior of the microbial communities in foods is of most importance for the production of high-quality and safe products. High-throughput sequencing (HTS) provides advanced approaches to determine food’s microbial community composition and structure. The aim of the present study was to determine the impact of different HP treatments on the microbial load and bacterial diversity of sous-vide Atlantic cod. Sous-vide cooking at 57.1 C for 30 min followed by HP treatment at 500 MPa for 8 min reduced viable cell counts (total aerobic mesophiles) in the cod samples below detectable levels for 45 days of storage under refrigeration. In a second trial with cod cooked sous-vide at 52 C for 20 min followed by HP treatments at 300 or 600 MPa (with HP treatment temperatures of 22 C or 50 C for 4 or 8 min, depending on treatment), only the treatments at 600 MPa delayed bacterial growth for at least 30 days under refrigeration. The optimal HP conditions to improve the microbiological quality of sous-vide cod cooked at low temperatures were obtained at 600 MPa for 4 min at a pressurization temperature of 50 C. Bacterial diversity was studied in cod cooked sous-vide at 52 C for 20 min by HTS. In the absence of HP treatment, Proteobacteria was the main bacterial group. A succession of Pseudomonadaceae (Pseudomonas) and Enterobacteriaceae was observed during storage. Firmicutes had low relative abundances and were represented mainly by Anoxybacillus (early storage) and Carnobacterium (late storage). The HP-treated sous-vide cod showed the greatest differences from controls during late storage, with Aerococcus and Enterococcus as predominant groups (depending on the HP conditions). The application of HTS provided new insights on the diversity and dynamics of the bacterial communities of sous-vide cod, revealing the presence of bacterial genera not previously described in this food, such as Anoxybacillus. The significance of Anoxybacillus as a contaminant of seafoods should be further investigated.
Efficacy of extracorporeal shockwave therapy, compared to corticosteroid injections, on pain, plantar fascia thickness and foot function in patients with plantar fasciitis: A systematic review and meta-analysis
(SAGE PUBLICATIONS LTD, 2024-05-23) Cortes-Perez, Irene; Moreno-Montilla, Laura; Ibanez-Vera, Alfonso Javier; Diaz-Fernandez, Angeles; Obrero-Gaitan, Esteban; Lomas-Vega, Rafael
Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering
(MDPI, 2020) Mora Boza, Ana; López Ruiz, Elena; López Donaire, María Luisa; Jiménez, Gema; Rosa Aguilar, María; Marchal, Juan Antonio; Perdiz, Jose Luis; Vázquez Lasa, Blanca; San Román, Julio; Gálvez Martín, Patricia
In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant di erences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic e ect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an e ective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field.

RUJA: Repositorio Institucional de Producción Científica

Examinar

Examinando DCS-Artículos por Título