Cellular extracts from post-mortem human cardiac tissue direct cardiomyogenic differentiation of human adipose tissue-derived stem cells
Fecha
2013
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Elsevier
Resumen
Background aims. Human adipose tissue-derived stem cells (hASCs) can be easily (and inexpensively) expanded in culture,
and their high plasticity allows their conversion to different cell types. We study the potential capacity of postmortem cardiac
tissue to direct cardiac differentiation of hASCs in vitro. Methods. Cardiac tissue collected from autopsies was used to obtain
cell extracts and conditioned medium, and both approaches were tested for cardiac induction. Results. Gene expression
analyses proved that post-mortem human cardiac tissue maintains genetic integrity. hASCs exposed to the cell extracts or
conditioned medium for 2 weeks achieved the appearance of myotube-like structures and were positive for cardiac markers
such as sarcomeric a-actinin, cardiac troponin I and T and desmin as proved by immunofluorescence. In addition,
differentiated cells showed increased expression of cardiomyocyte-related genes analyzed by reverse transcriptase polymerase
chain reaction (GATA-4, myocyte-enhancer factor-2c, a-cardiac actin and cardiac troponin I). Conclusions. For the first time,
post-mortem human cardiac tissue was used to induce hASC differentiation into myocardial-like cells. The methodology
described here would serve as a useful model to obtain cardiomyocyte-like cells in vitro.
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cardiomyogenic differentiation, cell extract, conditioned medium, human adipose stem cells, postmortem tissue