A soft 3D polyacrylate hydrogel recapitulates the cartilage niche and allows growth-factor free tissue engineering of human articular cartilage
Fecha
2019
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Editor
Elsevier
Resumen
Cartilage degeneration or damage treatment is still a challenge, but, tissue engineering strategies, which
combine cell therapy strategies, which combine cell therapy and scaffolds, and have emerged as a
promising new approach. In this regard, polyurethanes and polyacrylates polymers have been shown
to have clinical potential to treat osteochondral injuries. Here, we have used polymer microarrays technology
to screen 380 different polyurethanes and polyacrylates polymers. The top polymers with potential
to maintain chondrocyte viability were selected, with scale-up studies performed to evaluate their
ability to support chondrocyte proliferation during long-term culture, while maintaining their characteristic
phenotype. Among the selected polymers, poly (methylmethacrylate-co-methacrylic acid), showed
the highest level of chondrogenic potential and was used to create a 3D hydrogel. Ultrastructural morphology,
microstructure and mechanical testing of this novel hydrogel revealed robust characteristics
to support chondrocyte growth. Furthermore, in vitro and in vivo biological assays demonstrated that
chondrocytes cultured on the hydrogel had the capacity to produce extracellular matrix similar to hyaline
cartilage, as shown by increased expression of collagen type II, aggrecan and Sox9, and the reduced
expression of the fibrotic marker’s collagen type I. In conclusion, hydrogels generated from poly
(methylmethacrylate-co-methacrylic acid) created the appropriate niche for chondrocyte growth and
phenotype maintenance and might be an optimal candidate for cartilage tissue-engineering applications.
Descripción
Palabras clave
Polyacrylate Poly (methylmethacrylate-co-methacrylic acid) Polymer microarray Hydrogel Cartilage tissue engineering
Citación
Jiménez G*, Venkateswaran S*, López-Ruiz E*, Perán M, Pernagallo S, Díaz-Monchón JJ, Canadas RF, Antich C, Oliveira JM, Callanan A, Walllace R, Reis RL, Montañez E, Carrillo E, Bradley M, Marchal JA. A soft 3D polyacrylate hydrogel recapitulates the cartilage niche and allows growth-factor free tissue engineering of human articular cartilage. Acta Biomater. 2019 May;90:146-156. doi: 10.1016/j.actbio.2019.03.040.