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Title: A soft 3D polyacrylate hydrogel recapitulates the cartilage niche and allows growth-factor free tissue engineering of human articular cartilage
Authors: Jiménez, Gema
Venkateswaran, Seshasailam
López Ruiz, Elena
Perán, Macarena
Perigallo, Salvador
Díaz Manchón, Juan José
Canadas, Raplael F
Antich, Cristina
Oliviera, Joaquim M
Callanan, Anthony
Wallace, Robert
Reis, Rui L
Montanez, Elvira
Carrillo, Esmeralda
Bradley, Mark
Marchal, Juan Antonio
Abstract: Cartilage degeneration or damage treatment is still a challenge, but, tissue engineering strategies, which combine cell therapy strategies, which combine cell therapy and scaffolds, and have emerged as a promising new approach. In this regard, polyurethanes and polyacrylates polymers have been shown to have clinical potential to treat osteochondral injuries. Here, we have used polymer microarrays technology to screen 380 different polyurethanes and polyacrylates polymers. The top polymers with potential to maintain chondrocyte viability were selected, with scale-up studies performed to evaluate their ability to support chondrocyte proliferation during long-term culture, while maintaining their characteristic phenotype. Among the selected polymers, poly (methylmethacrylate-co-methacrylic acid), showed the highest level of chondrogenic potential and was used to create a 3D hydrogel. Ultrastructural morphology, microstructure and mechanical testing of this novel hydrogel revealed robust characteristics to support chondrocyte growth. Furthermore, in vitro and in vivo biological assays demonstrated that chondrocytes cultured on the hydrogel had the capacity to produce extracellular matrix similar to hyaline cartilage, as shown by increased expression of collagen type II, aggrecan and Sox9, and the reduced expression of the fibrotic marker’s collagen type I. In conclusion, hydrogels generated from poly (methylmethacrylate-co-methacrylic acid) created the appropriate niche for chondrocyte growth and phenotype maintenance and might be an optimal candidate for cartilage tissue-engineering applications.
Keywords: Polyacrylate Poly (methylmethacrylate-co-methacrylic acid) Polymer microarray Hydrogel Cartilage tissue engineering
Issue Date: 2019
metadata.dc.description.sponsorship: This work was supported by the Consejería de Economía, Innovación y Ciencia (Junta de Andalucía, excellence project number CTS-6568) and by the Ministerio de Economía, Industria y Competitividad (FEDER funds, project RTC-2016-5451-1). We acknowledge the Junta de Andalucía for providing a fellowship granted to G.J. and the MINECO for providing a post-doctoral fellowship to E.L-R (project RTC-2016-5451-1). MBR/SV thank the European Research Council (Advanced Grant ADREEM ERC-2013-340469).
Publisher: Elsevier
Citation: Jiménez G*, Venkateswaran S*, López-Ruiz E*, Perán M, Pernagallo S, Díaz-Monchón JJ, Canadas RF, Antich C, Oliveira JM, Callanan A, Walllace R, Reis RL, Montañez E, Carrillo E, Bradley M, Marchal JA. A soft 3D polyacrylate hydrogel recapitulates the cartilage niche and allows growth-factor free tissue engineering of human articular cartilage. Acta Biomater. 2019 May;90:146-156. doi: 10.1016/j.actbio.2019.03.040.
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