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Titre: Antimicrobial Resistance, Biocide Tolerance, and Bacterial Diversity of a Dressing Made from Coriander and Parsley after Application of Treatments Using High Hydrostatic Pressure Alone or in Combination with Moderate Heat
Auteur(s): Javier Rodríguez López
Maria José Grande Burgos
Rubén Pérez Pulido
Belén Iglesias Valenzuela
Antonio Gálvez
Rosario Lucas
Résumé: The effects of high-hydrostatic pressure (HP) treatments (450 and 600 megapascals, MPa, for 5 min at temperatures of 22 C and 50 C) on the microbiota of a coriander and parsley dressing was studied via culture-dependent and culture-independent approaches. Samples were refrigerated for 20 days, with periodic counts of the culture media supplemented with, or without, antimicrobials. HPtreated samples showed significantly lower viable cell counts compared to untreated controls. Only the control samples yielded bacterial growth on media with antimicrobials (imipenem, cefotaxime, benzalkonium chloride), including mostly Pseudomonas and Lactobacillus. Bacillus and Paenibacillus were identified from pressurized samples. Few isolates showed higher tolerance to some of the biocides tested. Pseudomonads showed outstanding resistance to meropenem and ceftazidime. According to high-throughput sequencing analysis, the microbiota of the dressing control samples changes during storage, with a reduction in the relative abundance of Proteobacteria and an increase in Firmicutes. The composition of the residual microbiota detected during storage was highly dependent on the pressure applied, and not on the treatment temperature
Mots-clés: antimicrobial resistance; high-hydrostatic pressure processing; bacterial diversity
Date de publication: 29-aoû-2022
metadata.dc.description.sponsorship: This research was funded by MINECO, grant number AGL2016-77374-R. J.R.L. was beneficiary of a research grant from the University of Jaen’s Plan de Apoyo a la Investigación. The APC was funded by University of Jaen.
Editeur: MDPI
Collection(s) :DCS-Artículos

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