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Title: Nitrated Fatty-Acids Distribution in Storage Biomolecules during Arabidopsis thaliana Development
Authors: Aranda-Caño, L.
Valderrama, R.
Chaki, M.
Begara-Morales, J.C.
Melguizo, M.
Barroso, J.B.
Abstract: The non-enzymatic interaction of polyunsaturated fatty acids with nitric oxide (NO) and de rived species results in the formation of nitrated fatty acids (NO2 -FAs). These signaling molecules can release NO, reversibly esterify with complex lipids, and modulate protein function through the post translational modification called nitroalkylation. To date, NO2 -FAs act as signaling molecules during plant development in plant systems and are involved in defense responses against abiotic stress conditions. In this work, the previously unknown storage biomolecules of NO2 -FAs in Arabidopsis thaliana were identified. In addition, the distribution of NO2 -FAs in storage biomolecules during plant development was determined, with phytosterol esters (SE) and TAGs being reservoir biomolecules in seeds, which were replaced by phospholipids and proteins in the vegetative, generative, and senescence stages. The detected esterified NO2 -FAs were nitro-linolenic acid (NO2 -Ln), nitro-oleic acid (NO2 -OA), and nitro-linoleic acid (NO2 -LA). The last two were detected for the first time in Arabidopsis. The levels of the three NO2 -FAs that were esterified in both lipid and protein storage biomolecules showed a decreasing pattern throughout Arabidopsis development. Esterification of NO2 -FAs in phospholipids and proteins highlights their involvement in both biomembrane dynamics and signaling processes, respectively, during Arabidopsis plant development
Keywords: NO2 -FAs; storage biomolecules; development; Arabidopsis thaliana; phospholipids; proteins
Issue Date: Sep-2022
metadata.dc.description.sponsorship: This research was funded by ERDF grants co-financed by the Spanish Ministry of Economy and Competitiveness (Project PGC2018-096405-B-I00); the Junta de Andalucía (group BIO286); the I+D+I project within the framework Programme of FEDER Andalucía 2014–2020 (Reference 1380901); the grants for I+D+I projects, on a competitive basis, within the scope of the Andalusian plan for research, development and innovation (Junta de Andalucía, PAIDI 2020, Reference: PY20_01002); and the funding for the recruitment of researchers under Action 9 and 10 of the Research Support Plan of the University of Jaén (2019–2020, R.02/10/2020; 2020–2021, R.01/01/2022).
Publisher: MDPI
Citation: Antioxidants (Basel). 2022. 11(10):1869
Appears in Collections:DBE-Artículos

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