Please use this identifier to cite or link to this item:
Title: Endogenous Biosynthesis of S-Nitrosoglutathione From Nitro-Fatty Acids in Plants
Authors: Mata-Pérez, C.
Padilla, M.N.
Sánchez-Calvo, B.
Begara-Morales, J.C.
Valderrama, R.
Chaki, M.
Aranda-Caño, L.
Moreno-González, D.
Molina-Díaz, A.
Barroso, J.B.
Abstract: Nitro-fatty acids (NO2-FAs) are novel molecules resulting from the interaction of unsaturated fatty acids and nitric oxide (NO) or NO-related molecules. In plants, it has recently been described that NO2-FAs trigger an antioxidant and a defence response against stressful situations. Among the properties of NO2-FAs highlight the ability to release NO therefore modulating specific protein targets through post-translational modifications (NO-PTMs). Thus, based on the capacity of NO2-FAs to act as physiological NO donors and using high-accuracy mass-spectrometric approaches, herein, we show that endogenous nitro-linolenic acid (NO2-Ln) can modulate S nitrosoglutathione (GSNO) biosynthesis in Arabidopsis. The incubation of NO2-Ln with GSH was analyzed by LC-MS/MS and the in vitro synthesis of GSNO was noted. The in vivo confirmation of this behavior was carried out by incubating Arabidopsis plants with 15N-labeled NO2-Ln throughout the roots, and 15N-labeled GSNO (GS15NO) was detected in the leaves. With the aim to go in depth in the relation of NO2-FA and GSNO in plants, Arabidopsis alkenal reductase mutants (aer mutants) which modulate NO2-FAs levels were used. Our results constitute the first evidence of the modulation of a key NO biological reservoir in plants (GSNO) by these novel NO2-FAs, increasing knowledge about S-nitrosothiols and GSNO-signaling pathways in plants.
Keywords: nitro-fatty acids, nitric oxide, S-nitrosoglutathione, S-nitrosothiols, NO-signaling, nitric oxide donor, Arabidopsis, alkenal reductase
Issue Date: Jun-2020
metadata.dc.description.sponsorship: This work was supported by an ERDF grant cofinanced by the Ministry of Economy and Competitiveness FIGURE 7 | Modulation of GSNO-signalling pathway by NO2-Ln in Arabidopsis leaves. NO2-Ln is up-taken by the root system and transported to plant leaves. Once in these organs, NO2-Ln can release nitric oxide (NO) and mediate the S-nitrosation of abundant glutathione (GSH) present in plant leaves and leading to the formation of S-nitrosoglutathione (GSNO). The generation of this low-molecular weight S-nitrosothiol (SNO) from NO2-Ln can affect the SNO-signaling pathway by modulating the transport and storage of NO, the response to several (a)biotic stress conditions or mediating the ability of SNO to perform post-translational modifications. Mata-Pe´ rez et al. Nitro-Fatty Acids Generates S-Nitrosogluthatione Frontiers in Plant Science | 10 June 2020 | Volume 11 | Article 962 (project PGC2018-096405-B-100), I+D+i Projects in the framework of the Andalusia 2014-2020 ERDF Operational Programme (Reference 1263509), Funding for the recruitment of researchers under the Action 10 of the Research Support Plan of the University of Jaén (2019-2020; R.02/10/2020) and the Junta de Andalucía (group BIO286) in Spain. LC-MS/MS technical and human support provided by CICT of Universidad de Jaé n (UJA, MINECO, Junta de Andalućıa, FEDER) is gratefully acknowledged.
Citation: Front Plant Sci. 2020. 11:962
Appears in Collections:DBE-Artículos

Files in This Item:
File Description SizeFormat 
Frontiers in Plant Science 2020.pdf1,03 MBAdobe PDFView/Open

This item is protected by original copyright