Please use this identifier to cite or link to this item: https://hdl.handle.net/10953/1888
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dc.contributor.authorGajdosechova, L-
dc.contributor.authorKrskova, K-
dc.contributor.authorSegarra, A-
dc.contributor.authorSpolcova, A-
dc.contributor.authorSuski, M-
dc.contributor.authorOlszanecki, R-
dc.contributor.authorZorad, S-
dc.date.accessioned2024-01-31T23:24:44Z-
dc.date.available2024-01-31T23:24:44Z-
dc.date.issued2014-03-
dc.identifier.citationGajdosechova L, Krskova K, Segarra AB, Spolcova A, Suski M, Olszanecki R, Zorad S. Hypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue. J Endocrinol. 2014 Feb 10;220(3):333-43. doi: 10.1530/JOE-13-0417.es_ES
dc.identifier.issn0022-0795es_ES
dc.identifier.other10.1530/JOE-13-0417es_ES
dc.identifier.urihttps://joe.bioscientifica.com/view/journals/joe/220/3/333.xmles_ES
dc.identifier.urihttps://hdl.handle.net/10953/1888-
dc.description.abstractThe metabolic action of oxytocin has recently been intensively studied to assess the ability of the peptide to regulate energy homeostasis. Despite the obvious weight-reducing effect of oxytocin observed in experimental studies, plasma oxytocin levels were found to be unchanged or even elevated in human obesity. The aim of our study was to evaluate the changes in the oxytocin system in Zucker rats, an animal model closely mirroring morbid obesity in humans. Plasma oxytocin levels were measured in obese Zucker rats and lean controls by enzyme immunoassay after plasma extraction. The expression of oxytocin and oxytocin receptor (OXTR) was assessed at the mRNA and protein levels by quantitative real-time PCR and immunoblotting respectively. Plasma and tissue activity of oxytocinase, the main enzyme involved in oxytocin degradation, were measured by fluorometric assay using an arylamide derivate as the substrate. Obese Zucker rats displayed a marked reduction in plasma oxytocin levels. Elevated liver and adipose tissue oxytocinase activity was noticed in obese Zucker rats. Hypothalamic oxytocin gene expression was not altered by the obese phenotype. OXTR mRNA and protein levels were upregulated in the adipose tissue of obese animals in contrast to the reduced OXTR protein levels in skeletal muscle. Our results show that obesity is associated with reduced plasma oxytocin due to increased peptide degradation by liver and adipose tissue rather than changes in hormone synthesis. This study highlights the importance of the oxytocin system in the pathogenesis of obesity and suggests oxytocinase inhibition as a candidate approach in the therapy of obesity.es_ES
dc.description.sponsorshipThis work was supported by the grants APVV 0028-10 and CE NOREG (Slovakia) and the National Science Centre 2011/01M/NZ04/03752 (Poland).es_ES
dc.language.isoenges_ES
dc.publisherBIOSCIENTIFICA LTDSTARLING HOUSE, 1600 BRISTOL PARKWAY N, BRISTOL BS34 8YU, ENGLANDes_ES
dc.relation.ispartofJournal of endocrinologyes_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleHypooxytocinaemia in obese Zucker rats relates to oxytocin degradation in liver and adipose tissue.es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.subject.udcEndocrinology & Metabolismes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES
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