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Title: Tolerance of olive (Olea europaea) cv Frantoio to Verticillium dahliae relies on both basal and pathogen-induced differential transcriptomic responses
Authors: Leyva-Pérez, M
Jiménez-Ruiz, J
Gómez-Lama Cabanás, C
Valverde-Corredor, A
Barroso, JB
Luque, F
Mercado-Blanco, J
Abstract: Verticillium wilt of olive (VWO) is one of the most serious biotic constraints for this tree crop. Our knowledge of the genetics of the tolerance/resistance to this disease is very limited. Here we show that tolerance of the cv Frantoio relies on both basal and early pathogen-induced differential transcriptomic responses. A comparative transcriptomic analysis (RNA-seq) was conducted in root tissues of cvs Frantoio (VWO-tolerant) and Picual (VWO-susceptible). RNA samples originated from roots of inoculated olive plants during the early infection stages by Verticillium dahliae (highly virulent, defoliating pathotype). A huge number of differentially expressed genes (DEGs) were found between 'Frantoio' and 'Picual' (27 312 unigenes) in the absence of the pathogen. Upon infection with V. dahliae, 'Picual' and 'Frantoio' plants responded differently too. In the early infection stages, four clusters of DEGs could be identified according to their time-course expression patterns. Among others, a pathogenesis-related protein of the Bet v I family and a dirigent-like protein involved in lignification, and several BAK1, NHL1, reactive oxygen species stress response and BAM unigenes showed noticeable differences between cultivars. Tolerance of 'Frantoio' plants to VWO is a consequence of a complex and multifaceted process which involves many plant traits.
Keywords: RNA-seq; defence response; defoliating pathotype; olive transcriptome; tolerance; vascular pathogen; verticillium wilt of olive.
Issue Date: 2018
metadata.dc.description.sponsorship: This work was supported by grant AGR-5948 from the Junta de Andalucía (Consejería de Economía, Innovación y Ciencia) and grants AGL2009-07275 and AGL2016-75729 from the Ministerio de Economía y Competitividad (MINECO)/Agencia Estatal de Investigación (co-financed by the European Regional Development Fund, ERDF). Technical and human support provided by CICT of the Universidad de Jaén (UJA, MINECO, Junta de Andalucía, FEDER) is gratefully acknowledged.
Citation: New Phytol . 2018 Jan;217(2):671-686.
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