RUJA: Repositorio Institucional de Producción Científica

 

Immunohistochemistry of neuronal nitric oxide synthase and protein nitration in the striatum of the aged rat

dc.contributor.authordel-Moral, María Luisa
dc.contributor.authorEsteban-Ruiz, Francisco José
dc.contributor.authorHernández-Cobo, Raquel
dc.contributor.authorBlanco-Ruiz, Santos
dc.contributor.authorMolina-Ortega, Francisco Javier
dc.contributor.authorMartínez-Lara, Esther
dc.contributor.authorSiles, Eva
dc.contributor.authorViedma, Gloria
dc.contributor.authorRuiz, Alharilla
dc.contributor.authorPedrosa-Raya, Juan Ángel
dc.contributor.authorPeinado-Herreros, María Ángeles
dc.date.accessioned2024-01-31T23:14:24Z
dc.date.available2024-01-31T23:14:24Z
dc.date.issued2004
dc.description.abstractTo ascertain the possible implications of the nitric oxide (NO● ) producing system in striatal senescence, and by using immunohistochemistry and image-processing approaches, we describe the presence of the enzyme nitric oxide synthase (NOS), the NADPH-diaphorase (NADPH-d) histochemical marker, and nitrotyrosine-derived complexes (N-Tyr) in the striatum of adult and aged rats. The results showed neuronal NOS immunoreactive (nNOS-IR) aspiny medium-sized neurons and nervous fibres in both age groups, with no variation in the percentage of immunoreactive area but a significant decrease in the intensity and in the number of somata with age, which were not related to the observed increase with age of the striatal bundles of the white matter. In addition, NADPH-d activity was detected in neurons with morphology similar to that of the nNOS-IR cells; a decrease in the percentage of area per field and in the number of cells, but an increase in the intensity of staining for the NADPH-d histochemical marker, were detected with age. The number of neuronal NADPH-d somata was higher than for the nNOS-IR ones in both age groups. Moreover, N-Tyr-IR complexes were observed in cells (neurons and glia) and fibres, with a significant increase in the percentage of the area of immunoreaction, related to the increase of white matter, but a decrease in intensity for the aged group. On the other hand, we did not detect the inducible isoform (iNOS) either in adult or in aged rats. Taken together, these results support the contention that NADPH-d staining is not such an unambiguous marker for nNOS, and that increased protein nitration may participate in striatal aging.
dc.identifier.issn1059-910Xes_ES
dc.identifier.otherhttps://doi.org/10.1002/jemt.20081es_ES
dc.identifier.urihttps://hdl.handle.net/10953/1830
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sons, Inc.es_ES
dc.relation.ispartofMicrosc. Res. Tech. [2004];[64]:[304-311]es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectBraines_ES
dc.subjectImage processinges_ES
dc.subjectNADPH-diaphorasees_ES
dc.subjectNOSes_ES
dc.titleImmunohistochemistry of neuronal nitric oxide synthase and protein nitration in the striatum of the aged rates_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones_ES

Archivos

Bloque original

Mostrando 1 - 1 de 1
Cargando...
Miniatura
Nombre:
AIN006 · Del Moral et al. Q1 JCR · 2004.pdf
Tamaño:
336.24 KB
Formato:
Adobe Portable Document Format
Descripción:
Published online in Wiley InterScience (www.interscience.wiley.com).

Bloque de licencias

Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.98 KB
Formato:
Item-specific license agreed upon to submission
Descripción:

Colecciones