Nitrated Fatty-Acids Distribution in Storage Biomolecules during Arabidopsis thaliana Development
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Fecha
2022-09
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MDPI
Resumen
The non-enzymatic interaction of polyunsaturated fatty acids with nitric oxide (NO) and de rived species results in the formation of nitrated fatty acids (NO2
-FAs). These signaling molecules can
release NO, reversibly esterify with complex lipids, and modulate protein function through the post translational modification called nitroalkylation. To date, NO2
-FAs act as signaling molecules during
plant development in plant systems and are involved in defense responses against abiotic stress
conditions. In this work, the previously unknown storage biomolecules of NO2
-FAs in Arabidopsis
thaliana were identified. In addition, the distribution of NO2
-FAs in storage biomolecules during plant
development was determined, with phytosterol esters (SE) and TAGs being reservoir biomolecules
in seeds, which were replaced by phospholipids and proteins in the vegetative, generative, and
senescence stages. The detected esterified NO2
-FAs were nitro-linolenic acid (NO2
-Ln), nitro-oleic
acid (NO2
-OA), and nitro-linoleic acid (NO2
-LA). The last two were detected for the first time in
Arabidopsis. The levels of the three NO2
-FAs that were esterified in both lipid and protein storage
biomolecules showed a decreasing pattern throughout Arabidopsis development. Esterification of
NO2
-FAs in phospholipids and proteins highlights their involvement in both biomembrane dynamics
and signaling processes, respectively, during Arabidopsis plant development
Descripción
Palabras clave
NO2 -FAs; storage biomolecules; development; Arabidopsis thaliana; phospholipids; proteins
Citación
Antioxidants (Basel). 2022. 11(10):1869