ANÁLISIS CITOGENÉTICO Y MOLECULAR EN ESPECIES DEL GÉNERO TALPA (INSECTIVORA;TALPIDAE)
Fecha
2023-11-10
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Jaén : Universidad de Jaén
Resumen
Hemos descrito por primera vez el cariotipo de T. aquitania. Salvo diferencias en el cromosoma Y y en algunos pares autosómicos, demostramos su similitud con los cariotipos de otras especies del género. La comparación del cariotipo de tres especies de Talpa mediante FISH y “chromosome painting”, demuestra que las secuencias repetidas LINE1están ampliamente distribuidas, aunque se acumulan en regiones pericentroméricas y bloques heterocromáticos de los cromosomas y en los cromosomas Y, los cuales están altamente conservados. Hemos caracterizado el satelitoma de T. aquitania, que representa el 1.24% del genoma y contiene 16 familias diferentes, en su mayoría presentes con distinta abundancia en otras especies de Talpa. Mediante FISH, comprobamos que algunos ADNsat se localizan preferentemente en regiones heterocromáticas. Hemos descrito los mitogenomas de Talpa occidentalis y Talpa aquitania, demostrando que se diferencian en la repetición de la región D-Loop, y son similares a otros mitogenomas de mamíferos.
We have described the karyotype of T. aquitania for the first time. Apart from differences in the Y chromosome and some autosomal pairs, we have demonstrated its similarity with the karyotypes of other species in the genus. Comparison of the karyotype of three Talpa species using FISH and chromosome painting showed that LINE1 repeated sequences are widely distributed, although they accumulate in pericentromeric regions, heterochromatic blocks of chromosomes, and Y chromosomes, which are highly conserved. We have characterized the T. aquitania satellitome, which represents 1.24% of the genome and contains 16 different families, mostly present with different abundance in other Talpa species. Using FISH, we have verified that some satellite DNA is preferably located in heterochromatic regions. We have described the mitogenomes of Talpa occidentalis and Talpa aquitania, demonstrating that they differ in the repetition of the D-Loop region and are similar to other mammalian mitogenomes.
We have described the karyotype of T. aquitania for the first time. Apart from differences in the Y chromosome and some autosomal pairs, we have demonstrated its similarity with the karyotypes of other species in the genus. Comparison of the karyotype of three Talpa species using FISH and chromosome painting showed that LINE1 repeated sequences are widely distributed, although they accumulate in pericentromeric regions, heterochromatic blocks of chromosomes, and Y chromosomes, which are highly conserved. We have characterized the T. aquitania satellitome, which represents 1.24% of the genome and contains 16 different families, mostly present with different abundance in other Talpa species. Using FISH, we have verified that some satellite DNA is preferably located in heterochromatic regions. We have described the mitogenomes of Talpa occidentalis and Talpa aquitania, demonstrating that they differ in the repetition of the D-Loop region and are similar to other mammalian mitogenomes.
Descripción
Palabras clave
Genoma mitocondrial, análisis citogenético, satelitoma, secuencias repetidas, Talpidae
Citación
p.[http://hdl.handle.net/10953/]