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Differential transcriptomic analysis by RNA-Seq of GSNO-responsive genes between Arabidopsis roots and leaves

dc.contributor.authorBegara-Morales, Juan Carlos
dc.contributor.authorSánchez-Calvo, Beatriz
dc.contributor.authorLuque-Vázquez, Francisco
dc.contributor.authorLeyva-Pérez, María O
dc.contributor.authorLeterrier, Marina
dc.contributor.authorCorpas, Francisco Javier
dc.contributor.authorBarroso-Albarracín, Juan Bautista
dc.date.accessioned2025-01-27T00:04:22Z
dc.date.available2025-01-27T00:04:22Z
dc.date.issued2014-06
dc.descriptionThis is the peer reviewed version of the following article: [Begara-Morales JC, Sánchez-Calvo B, Luque F, Leyva-Pérez MO, Leterrier M, Corpas FJ, Barroso JB. Differential transcriptomic analysis by RNA-Seq of GSNO-responsive genes between Arabidopsis roots and leaves. Plant Cell Physiol. 2014 Jun;55(6):1080-95. doi: 10.1093/pcp/pcu044.] This accepted version of the article may be used for non-commercial purposes in accordance with Oxford Academic terms and conditions for use of self-archived versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Oxford Academic or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Oxford´s version of record on Plant and Cell Physiology online library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Oxford online library must be prohibited.es_ES
dc.description.abstractS-Nitrosoglutathione (GSNO) is a nitric oxide-derived molecule that can regulate protein function by a post-translational modification designated S-nitrosylation. GSNO has also been detected in different plant organs under physiological and stress conditions, and it can also modulate gene expression. Thirty-day-old Arabidopsis plants were grown under hydroponic conditions, and exogenous 1 mM GSNO was applied to the root systems for 3 h. Differential gene expression analyses were carried out both in roots and in leaves by RNA sequencing (RNA-seq). A total of 3,263 genes were identified as being modulated by GSNO. Most of the genes identified were associated with the mechanism of protection against stress situations, many of these having previously been identified as target genes of GSNO by array-based methods. However, new genes were identified, such as that for methionine sulfoxide reductase (MSR) in leaves or different miscellaneous RNA (miscRNA) genes in Arabidopsis roots. As a result, 1,945 GSNO-responsive genes expressed differently in leaves and roots were identified, and 114 of these corresponded exclusively to one of these organs. In summary, it is demonstrated that RNA-seq extends our knowledge of GSNO as a signaling molecule which differentially modulates gene expression in roots and leaves under non-stress conditions.es_ES
dc.identifier.citationBegara-Morales JC, Sánchez-Calvo B, Luque F, Leyva-Pérez MO, Leterrier M, Corpas FJ, Barroso JB. Differential transcriptomic analysis by RNA-Seq of GSNO-responsive genes between Arabidopsis roots and leaves. Plant Cell Physiol. 2014 Jun;55(6):1080-95. doi: 10.1093/pcp/pcu044.es_ES
dc.identifier.issn0032-0781es_ES
dc.identifier.other10.1093/pcp/pcu044es_ES
dc.identifier.urihttps://academic.oup.com/pcp/article-abstract/55/6/1080/2756401?redirectedFrom=fulltext&login=falsees_ES
dc.identifier.urihttps://hdl.handle.net/10953/4396
dc.language.isoenges_ES
dc.publisherOxford Academices_ES
dc.relation.ispartofPlant and Cell Physiologyes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectArabidopsises_ES
dc.subjectMassively parallel sequencinges_ES
dc.subjectMethionine sulfoxide reductasees_ES
dc.subjectNitric oxidees_ES
dc.subjectPlant singaling mechanismses_ES
dc.subjectRNA-seqes_ES
dc.subjectS-nitrosoglutathionees_ES
dc.titleDifferential transcriptomic analysis by RNA-Seq of GSNO-responsive genes between Arabidopsis roots and leaveses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones_ES

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