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Biological Implications of a Stroke Therapy Based in Neuroglobin Hyaluronate Nanoparticles. Neuroprotective Role and Molecular Bases

dc.contributor.authorPeinado-Herreros, María Ángeles
dc.contributor.authorOvelleiro, David
dc.contributor.authordel-Moral, María Luisa
dc.contributor.authorHernández-Cobo, Raquel
dc.contributor.authorMartínez-Lara, Esther
dc.contributor.authorSiles, Eva
dc.contributor.authorPedrajas, José Rafael
dc.contributor.authorGarcía-Martín, María Luisa
dc.contributor.authorCaro, Carlos
dc.contributor.authorPeralta, Sebastián
dc.contributor.authorMorales-Hernández, María Encarnación
dc.contributor.authorRuiz-Martínez, María Adolfina
dc.contributor.authorBlanco-Ruiz, Santos
dc.date.accessioned2024-01-29T08:41:00Z
dc.date.available2024-01-29T08:41:00Z
dc.date.issued2022
dc.description.abstractExogenous neuroprotective protein neuroglobin (Ngb) cannot cross the blood–brain barrier. To overcome this difficulty, we synthesized hyaluronate nanoparticles (NPs), able to deliver Ngb into the brain in an animal model of stroke (MCAO). These NPs effectively reached neurons, and were microscopically identified after 24 h of reperfusion. Compared to MCAO non-treated animals, those treated with Ngb-NPs showed survival rates up to 50% higher, and better neurological scores. Tissue damage improved with the treatment, but no changes in the infarct volume or in the oxidative/nitrosative values were detected. A proteomics approach (p-value < 0.02; fold change = 0.05) in the infarcted areas showed a total of 219 proteins that significantly changed their expression after stroke and treatment with Ngb-NPs. Of special interest, are proteins such as FBXO7 and NTRK2, which were downexpressed in stroke, but overexpressed after treatment with Ngb-NPs; and ATX2L, which was overexpressed only under the effect of Ngb. Interestingly, the proteins affected by the treatment with Ngb were involved in mitochondrial function and cell death, endocytosis, protein metabolism, cytoskeletal remodeling, or synaptic function, and in regenerative processes, such as dendritogenesis, neuritogenesis, or sinaptogenesis. Consequently, our pharmaceutical preparation may open new therapeutic scopes for stroke and possibly for other neurodegenerative pathologies.
dc.identifier.issn1422-0067es_ES
dc.identifier.otherhttps://doi.org/10.3390/ijms23010247es_ES
dc.identifier.urihttps://hdl.handle.net/10953/1700
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.relation.ispartofInt. J. Mol. Sci. [2022];[23]:[247]es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectNeuroglobin
dc.subjectHyaluronate nanoparticles
dc.subjectStroke
dc.subjectMCAO
dc.subjectProteomics
dc.subjectNeuroprotection
dc.titleBiological Implications of a Stroke Therapy Based in Neuroglobin Hyaluronate Nanoparticles. Neuroprotective Role and Molecular Baseses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES

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