RUJA: Repositorio Institucional de Producción Científica

 

Crosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cells

dc.contributor.authorCalahorra, Jesús
dc.contributor.authorMartínez-Lara, Esther
dc.contributor.authorGranadino-Roldán, José Manuel
dc.contributor.authorMartí, Juan Manuel
dc.contributor.authorCañuelo, Ana
dc.contributor.authorBlanco-Ruiz, Santos
dc.contributor.authorOliver, Francisco Javier
dc.contributor.authorSiles, Eva
dc.date.accessioned2024-01-29T08:48:37Z
dc.date.available2024-01-29T08:48:37Z
dc.date.issued2020
dc.description.abstractOlive oil intake has been linked with a lower incidence of breast cancer. Hypoxic microenvironment in solid tumors, such as breast cancer, is known to play a crucial role in cancer progression and in the failure of anticancer treatments. HIF-1 is the foremost effector in hypoxic response, and given that hydroxytyrosol (HT) is one of the main bioactive compounds in olive oil, in this study we deepen into its modulatory role on HIF-1. Our results in MCF-7 breast cancer cells demonstrate that HT decreases HIF-1α protein, probably by downregulating oxidative stress and by inhibiting the PI3K/Akt/mTOR pathway. Strikingly, the expression of HIF-1 target genes does not show a parallel decrease. Particularly, adrenomedullin and vascular endothelial growth factor are up-regulated by high concentrations of HT even in HIF-1α silenced cells, pointing to HIF-1-independent mechanisms of regulation. In fact, we show, by in silico modelling and transcriptional analysis, that high doses of HT may act as an agonist of the aryl hydrocarbon receptor favoring the induction of these angiogenic genes. In conclusion, we suggest that the effect of HT in a hypoxic environment is largely affected by its concentration and involves both HIF-1 dependent and independent mechanisms.
dc.identifier.issn2045-2322es_ES
dc.identifier.otherhttps://doi.org/10.1038/s41598-020-63417-6es_ES
dc.identifier.urihttps://hdl.handle.net/10953/1735
dc.language.isoenges_ES
dc.publisherNature Researches_ES
dc.relation.ispartofSci Rep. [2020];[10]:[6361]es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.titleCrosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cellses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES

Archivos

Bloque original

Mostrando 1 - 1 de 1
Cargando...
Miniatura
Nombre:
AIN022 · Calahorra et al. Q1 JCR · 2020.pdf
Tamaño:
2.09 MB
Formato:
Adobe Portable Document Format
Descripción:

Bloque de licencias

Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.98 KB
Formato:
Item-specific license agreed upon to submission
Descripción:

Colecciones