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Development of a process for the production of L-aminoacids concentrates from microalgae by enzymatic hydrolysis

dc.contributor.authorRomero-García, Juan M.
dc.contributor.authorAcién, Francisco G.
dc.contributor.authorFernández-Sevilla, José M.
dc.date.accessioned2025-01-30T07:20:36Z
dc.date.available2025-01-30T07:20:36Z
dc.date.issued2012-02-28
dc.descriptionD1 Q1, 1/12 Agricultural engineering, 2012 IF = 4.75es_ES
dc.description.abstractA process for the production of L-amino-acids concentrates from microalgae biomass by enzymatic hydrolysis has been developed. The process includes pre-treatment for cell-disruption, enzymatic hydro- lysis and final separation by centrifugation. Thermal and mechanical cell-disruption methods have been tested, selecting mechanical disruption using bead milling for 30 min. The enzymatic hydrolysis was done using the commercial enzymes Alcalase and Flavourzyme. Maximum hydrolysis was obtained for biomass concentrations under 270 g/l and previous additional treatment with Viscozyme, reaching a 42% hydrolysis. Repeated reaction steps increased the hydrolysis from 42% (4 h) with a single step to 59% (8 h) after two successive steps. Further increase of the number of steps had a meagre impact on the global yield. The process widens the portfolio of products that can be obtained from microalgae bio- mass and is a new possibility to enhance the economic viability of microalgae-based biofuels production processes.es_ES
dc.description.sponsorshipThis research was partially funded by the grant CTQ2008- 06741-CO2-01/PPQ, Ministerio de Ciencia e Innovación, Gobierno de España, CENIT-CO2 project with financing by ENDESA S.A. and Ministerio de Industria (CDTI), and Junta de Andalucía and Plan Andaluz de Investigación (BIO 173).es_ES
dc.identifier.citationRomero García, J.M., Acién Fernández, F.G., Fernández Sevilla, J.M. Development of a process for the production of l-amino-acids concentrates from microalgae by enzymatic hydrolysis.es_ES
dc.identifier.issn0960-8524es_ES
dc.identifier.other10.1016/j.biortech.2012.02.094es_ES
dc.identifier.urihttps://doi.org/10.1016/j.biortech.2012.02.094es_ES
dc.identifier.urihttps://hdl.handle.net/10953/4526
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.ispartofBioresource Technology 2012; 112: 164– 170es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectMicroalgae biomasses_ES
dc.subjectFree-amino-acids concentrateses_ES
dc.subjectProtein hydrolysises_ES
dc.subjectEnzymatic hydrolysises_ES
dc.titleDevelopment of a process for the production of L-aminoacids concentrates from microalgae by enzymatic hydrolysises_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones_ES

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Development of a process for the production of l-amino-acids concentrates from microalgae by enzymatic hydrolysis.pdf
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Development of a process for the production of l-amino-acids concentrates from microalgae by enzymatic hydrolysis. Romero García, J.M., Acién Fernández, F.G., Fernández Sevilla, J.M. Bioresource Technology, 2012, 112, pp. 164– 170

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