Development of a process for the production of L-aminoacids concentrates from microalgae by enzymatic hydrolysis
Fecha
2012-02-28
Título de la revista
ISSN de la revista
Título del volumen
Editor
Elsevier
Resumen
A process for the production of L-amino-acids concentrates from microalgae biomass by enzymatic hydrolysis has been developed. The process includes pre-treatment for cell-disruption, enzymatic hydro- lysis and final separation by centrifugation. Thermal and mechanical cell-disruption methods have been tested, selecting mechanical disruption using bead milling for 30 min. The enzymatic hydrolysis was done using the commercial enzymes Alcalase and Flavourzyme. Maximum hydrolysis was obtained for biomass concentrations under 270 g/l and previous additional treatment with Viscozyme, reaching a 42% hydrolysis. Repeated reaction steps increased the hydrolysis from 42% (4 h) with a single step to 59% (8 h) after two successive steps. Further increase of the number of steps had a meagre impact on the global yield. The process widens the portfolio of products that can be obtained from microalgae bio- mass and is a new possibility to enhance the economic viability of microalgae-based biofuels production processes.
Descripción
D1 Q1, 1/12 Agricultural engineering, 2012 IF = 4.75
Palabras clave
Microalgae biomass, Free-amino-acids concentrates, Protein hydrolysis, Enzymatic hydrolysis
Citación
Romero García, J.M., Acién Fernández, F.G., Fernández Sevilla, J.M. Development of a process for the production of l-amino-acids concentrates from microalgae by enzymatic hydrolysis.