Cardiomyogenic differentiation potential of human endothelial progenitor cells isolated from patients with myocardial infarction
Fecha
2014
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Editor
Elsevier
Resumen
Background aims. Endothelial progenitor cells (EPCs) are known to play a beneficial role by promoting postnatal vasculogenesis
in pathological events, such as ischemic heart disease and peripheral artery disease. However, little is known about
the potential of EPCs to restore heart damage tissue. We compared the cardiac differentiation capacity of EPCs isolated from
peripheral blood of patients with acute myocardial infarction (AMI) with EPCs obtained from umbilical cord blood (UCB).
Methods. EPCs from both origins were isolated by density gradient centrifugation and characterized through the use of
endothelial markers (UEA-1lectin, CD133 and KDR) and endothelial cell colony-forming unit assay. Cardiac differentiation
capacity of EPCs was assessed by immunofluorescence and reverse transcriptaseepolymerase chain reaction after 5-
azacytidine (5-aza) induction. Results. No significant differences were observed between the number of endothelial cell
colony-forming units in peripheral blood of patients with AMI and samples from UCB. Moreover, 5-aza induced the
appearance of myotube-like structures and the positive expression of sarcomeric a-actinin, cardiac troponin I and T and
desmin in a similar pattern for both cell sources, which indicates a comparable acquisition of a cardiac-like phenotype.
Conclusions. For the first time, we have compared, in vitro, the cardiomyogenic potential of EPCs derived from patients with
AMI with UCB-derived EPCs. Our data indicate that EPCs obtained from both origins have similar plasticity and functions
and suggest a potential therapeutic efficacy in cardiac cell therapy.
Descripción
Palabras clave
acute myocardial infarction, cardiomyocyte differentiation, endothelial progenitor cells, umbilical cord blood
Citación
López-Ruiz E, Perán M, Picón-Ruiz M, García MA, Carrillo E, Jiménez-Navarro M, Hernández MC, Prat I, De Teresa E, Marchal JA. Cardiomyogenic differentiation potential of human endothelial progenitor cells isolated from patients with myocardial infarction. Cytotherapy. 2014 Sep;16(9):1229-37. doi: 10.1016/j.jcyt.2014.05.012. Epub 2014 Jun 23.