Glutamil aminopeptidasa en orina, en microvesículas y en exosomas como marcador de la nefrotoxicidad inducida por el cisplatino en ratas
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2017-07-07
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Jaén : Universidad de Jaén
Resumen
El objetivo principal de esta Tesis Doctoral es investigar si la excreción de glutamil aminopeptidasa (GluAp) es un marcador de la nefrotoxicidad inducida por el cisplatino en ratas. En un primer experimento se comprobó que la excreción temprana de GluAp, determinada por fluorimetría cinética, ELISA e inmunoblotting se encontraba aumentada en las ratas tratadas con cisplatino, y tenía un valor predictivo sobre la disfunción renal. En otro trabajo se demostró que la GluAp presente en las fracciones microvesicular y exosómica urinarias también correlacionaba con la disfunción renal, tanto si los datos se normalizaban por excreción total diaria, por concentración de creatinina urinaria o por concentración de proteína total en cada fracción. Se concluye que la excreción de GluAp en orina es un marcador del daño renal producido por el cisplatino en ratas que puede ser útil para evaluar el daño tubular renal de manera independiente a la excreción de creatinina.
The main objective of this Doctoral Thesis is to investigate whether the excretion of glutamyl aminopeptidase (GluAp), is a marker of cisplatin-induced nephrotoxicity in rats. In a first experiment, the early excretion of GluAp as determined by kinetic fluorimetry, ELISA and immunoblotting was found to be increased in cisplatin-treated rats and it showed a predictive value over renal dysfunction. In other work, it was demonstrated that GluAp in microvesicular and exosomal urinary fractions was also correlated with renal dysfunction if data were normalized by daily total excretion, by concentration of urinary creatinine or by total protein in each fraction. It is concluded that excretion of GluAp in urine is a marker of renal damage induced by cisplatin in rats that can be useful to evaluate renal tubular damage independently of creatinine excretion.
The main objective of this Doctoral Thesis is to investigate whether the excretion of glutamyl aminopeptidase (GluAp), is a marker of cisplatin-induced nephrotoxicity in rats. In a first experiment, the early excretion of GluAp as determined by kinetic fluorimetry, ELISA and immunoblotting was found to be increased in cisplatin-treated rats and it showed a predictive value over renal dysfunction. In other work, it was demonstrated that GluAp in microvesicular and exosomal urinary fractions was also correlated with renal dysfunction if data were normalized by daily total excretion, by concentration of urinary creatinine or by total protein in each fraction. It is concluded that excretion of GluAp in urine is a marker of renal damage induced by cisplatin in rats that can be useful to evaluate renal tubular damage independently of creatinine excretion.
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Aminopeptidasas, exosomas, microvesículas, disfunción renal, cisplatino, Aminopeptidases, exosomes, microvesicles, renal dysfunction, cisplatin
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p.[http://hdl.handle.net/10953/]