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Differential chamber-specific expression and regulation of long non-coding RNAs during cardiac development

dc.contributor.authorGarcía-Padilla, Carlos
dc.contributor.authorDomínguez-Macías, Jorge Nicolás
dc.contributor.authorAránega, Amelia Eva
dc.contributor.authorFranco, Diego
dc.date.accessioned2025-01-28T22:47:00Z
dc.date.available2025-01-28T22:47:00Z
dc.date.issued2019-10
dc.description.abstractCardiovascular development is governed by a complex interplay between inducting signals such as Bmps and Fgfs leading to activation of cardiac specific transcription factors such as Nkx2.5, Mef2c and Srf that orchestrate the initial steps of cardiogenesis. Over the last decade we have witnessed the discovery of novel layers of gene regulation, i.e. post-transcriptional regulation exerted by non-coding RNAs. The function role of small non coding RNAs has been widely demonstrated, e.g. miR-1 knockout display several cardiovascular abnormalities during embryogenesis. More recently long non-coding RNAs have been also reported to modulate gene expression and function in the developing heart, as exemplified by the embryonic lethal phenotypes of Fendrr and Braveheart knock out mice, respectively. In this study, we investigated the differential expression profile during cardiogenesis of previously reported lncRNAs in heart development. Our data revealed that Braveheart, Fendrr, Carmen display a preferential adult expression while Miat, Alien, H19 preferentially display chamber-specific expression at embryonic stages. We also demonstrated that these lncRNAs are differentially regulated by Nkx2.5, Srf and Mef2c, Pitx2 > Wnt > miRNA signaling pathway and angiotensin II and thyroid hormone administration. Importantly isoform-specific expression and distinct nuclear vs cytoplasmic localization of Braveheart, Carmen and Fendrr during chamber morphogenesis is observed, suggesting distinct functional roles of these lncRNAs in atrial and ventricular chambers. Furthermore, we demonstrate by in situ hybridization a dynamic epicardial, myocardial and endocardial expression of H19 during cardiac development. Overall our data support novel roles of these lncRNAs in different temporal and tissue-restricted fashion during cardiogenesis.
dc.identifier.citationGarcía-Padilla C, Domínguez JN, Aránega AE, Franco D. Differential chamber-specific expression and regulation of long non-coding RNAs during cardiac development. Biochim Biophys Acta Gene Regul Mech. 2019 Oct;1862(10):194435. doi: 10.1016/j.bbagrm.2019.194435. Epub 2019 Nov 1. PMID: 31678627.es_ES
dc.identifier.issnOnline ISSN: 1876-4320Print ISSN: 1874-9399es_ES
dc.identifier.otherdoi: 10.1016/j.bbagrm.2019.194435es_ES
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1874939919301920?via%3Dihubes_ES
dc.identifier.urihttps://hdl.handle.net/10953/4465
dc.publisherElsevieres_ES
dc.relation.ispartofBiochim Biophys Acta Gene Regul Meches_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.titleDifferential chamber-specific expression and regulation of long non-coding RNAs during cardiac developmentes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones_ES

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