DBE-Artículos
URI permanente para esta colecciónhttps://hdl.handle.net/10953/111
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Examinando DBE-Artículos por Materia "Alkenal reductase"
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Ítem Endogenous Biosynthesis of S-Nitrosoglutathione From Nitro-Fatty Acids in Plants(FRONTIERS MEDIA SA, 2020-06) Mata-Pérez, Capilla; Padilla-Serrano, María Nieves; Sánchez-Calvo, Beatriz; Begara-Morales, Juan Carlos; Valderrama, Raquel; Chaki, Mounira; Aranda-Caño, Lorena; Moreno-González, David; Molina-Díaz, Antonio; Barroso-Albarracín, Juan BautistaNitro-fatty acids (NO2-FAs) are novel molecules resulting from the interaction of unsaturated fatty acids and nitric oxide (NO) or NO-related molecules. In plants, it has recently been described that NO2-FAs trigger an antioxidant and a defence response against stressful situations. Among the properties of NO2-FAs highlight the ability to release NO therefore modulating specific protein targets through post translational modifications (NO-PTMs). Thus, based on the capacity of NO2-FAs to act as physiological NO donors and using high-accuracy mass-spectrometric approaches, herein, we show that endogenous nitro-linolenic acid (NO2-Ln) can modulate S nitrosoglutathione (GSNO) biosynthesis in Arabidopsis. The incubation of NO2-Ln with GSH was analyzed by LC-MS/MS and the in vitro synthesis of GSNO was noted. The in vivo confirmation of this behavior was carried out by incubating Arabidopsis plants with 15N-labeled NO2-Ln throughout the roots, and 15N-labeled GSNO (GS15NO) was detected in the leaves. With the aim to go in depth in the relation of NO2-FA and GSNO in plants, Arabidopsis alkenal reductase mutants (aer mutants) which modulate NO2-FAs levels were used. Our results constitute the first evidence of the modulation of a key NO biological reservoir in plants (GSNO) by these novel NO2-FAs, increasing knowledge about S-nitrosothiols and GSNO signaling pathways in plants.