DBE-Artículos
URI permanente para esta colecciónhttps://hdl.handle.net/10953/111
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Examinando DBE-Artículos por Materia "Aging"
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Ítem Age-related changes of the nitric oxide system in the rat brain(Elsevier BV, 2002) Siles, Eva; Martínez-Lara, Esther; Cañuelo, Ana; Sánchez, Marta; Hernández-Cobo, Raquel; López-Ramos, Juan Carlos; del-Moral, María Luisa; Esteban-Ruiz, Francisco José; Blanco-Ruiz, Santos; Pedrosa-Raya, Juan ángel; Rodrigo, José; Peinado-Herreros, María ÁngelesThis work examines the age-related changes of the NO pathway in the central nervous system (CNS), analyzing nitric oxide synthase (NOS) isoform expression, the level of nitrotyrosine-modified proteins, and the NOS activity in the cerebral cortex, decorticated brain (basal ganglia, thalamus, hypothalamus, tegtum and tegmentum) and cerebellum of young, adult and aged rats. Our data demonstrate that the different NOS isoforms are not uniformly expressed across the CNS. In this sense, the nNOS and eNOS isoenzymes are expressed mainly in the cerebellum and decorticated brain, respectively, while the iNOS isoenzyme shows the highest level in cerebellum. Concerning age, in the cerebral cortex nNOS significantly increased its expression only in adult animals; meanwhile, in the cerebellum the eNOS expression decreased whereas iNOS increased in adult and aged rats. No age-related changes in any isoform were found in decorticated brain. NOS activity, determined by nitrate plus nitrite quantification, registered the highest levels in the cerebellum, where the significant increase detected with aging was probably related to iNOS activity. The number of nitrotyrosine-modified immunoreactive bands differed among regions; thus, the highest number was detected in the decorticated brain while the cerebellum showed the least number of bands. Finally, bulk protein nitration increased in cerebral cortex only in adult animal. No changes were found in the decorticated brain, and the decrease detected in the cerebellum of aged animals was not significant. According to these results, the NO pathway is differently modified with age in the three CNS regions analyzed.Ítem Glutathione S-transferase isoenzymatic response to aging in rat cerebral cortex and cerebellum(Elsevier Inc., 2003) Martínez-Lara, Esther; Siles, Eva; Hernández-Cobo, Raquel; Cañuelo, Ana; del-Moral, María Luisa; Jiménez, Ana; Blanco-Ruiz, Santos; López-Ramos, Juan Carlos; Esteban-Ruiz, Francisco José; Pedrosa-Raya, Juan Ángel; Peinado-Herreros, María ÁngelesAging is associated with increased oxidant generation. One mechanism involved in the defense of oxidative products is the family of glutathione transferases (GST). We have analyzed the activity, distribution and expression of GSTP1 and GSTA4 isoenzymes in the cerebral cortex and cerebellum of young, adult and aged rats. The total GST activity, measured with the universal substrate 1-chloro-2,4-dinitrobenzene (CDNB), increased only with the maturation process; however GSTA4 activity, using the specific substrate 4-hydroxynonenal (HNE), did show an age-dependent increase in both brain regions. Cellular location of GSTA4 in astrocytes was not changed except for young cerebral cortex and adult/aged cerebellum that also showed immunoreactivity in layer III pyramidal neurons and Bergman radial glia, respectively. Distribution of GSTP1 was similar among groups and only an increased number of positive oligodendrocytes was found in the Purkinje and granular layer of adult/aged cerebellum. The GSTA4 and GSTP1 expression increased from young to adult/aged brain and GSTA4 even augmented in the aged cerebral cortex. These results suggest a GST isoenzymatic response with aging, but above all with the maturation process.