Departamento de Biología Experimental
URI permanente para esta comunidadhttps://hdl.handle.net/10953/9
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Examinando Departamento de Biología Experimental por Materia "6 Ciencias aplicadas. Medicina. Tecnología."
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Ítem Celiac Immunogenic Potential of α-Gliadin Epitope Variants from Triticum and Aegilops Species(MDPI, 2019-01) Ruiz-Carnicer, Ángela; Comino-Montilla, Isabel; Segura, Veronica; Ozuna, Carmen Victoria; Moreno-Amador, María Lourdes; López-Casado, Miguel Ángel; Torres-López, María Isabel; Barro, Francisco; Sousa, CarolinaThis study investigates the immunotoxic potential of α-gliadins in wheat species and their implications for celiac disease (CD). Gluten, the primary structural protein complex in wheat, contains α-gliadins, which are rich in T-cell stimulating epitopes (DQ2.5-glia-α1, DQ2.5-glia-α2, and DQ2.5-glia-α3) that trigger immune responses in individuals with CD. The analysis spanned diploid, tetraploid, and hexaploid wheat species, focusing on the abundance and immunostimulatory capacity of canonical epitopes and their variants.The findings reveal that DQ2.5-glia-α1 and DQ2.5-glia-α3 are more prevalent than DQ2.5-glia-α2. Canonical DQ2.5-glia-α1 is notably abundant in genomes of the BBAADD, AA, and DD types, while DQ2.5-glia-α3 epitope variants are highly represented in BBAADD and BBAA wheats, despite a lower presence of the canonical form. Importantly, the introduction of a natural amino acid substitution (Q to H) at any position effectively neutralized the immunotoxicity of the epitopes without compromising wheat's functional properties.This work underscores the potential of targeted amino acid substitutions as a strategy to develop wheat varieties that are safer for individuals with CD while preserving their technological value.Ítem Dysregulation of the PD-1/PD-L1 pathway contributes to the pathogenesis of celiac disease(Nature group, 2019-06) Ponce de León, Candelaria; López-Casado, Miguel Ángel; Lorite, Pedro; Palomeque, Teresa; Torres, María IsabelThis study investigates changes in the programmed cell death 1 (PD-1)/PD-L1 pathway in celiac disease (CD), emphasizing its role in immune response regulation and its potential to enhance understanding of CD autoimmunity. The research highlights the importance of measuring PD-1 and PD-L1 levels in serum and intestinal biopsies of CD patients to explore potential links between these markers, autoimmune activity, inflammation, and disease progression.Ítem Expression of Elafin and CD200 as Immune Checkpoint Molecules Involved in Celiac Disease.(MDPI, 2024-01) Ponce de León, Candelaria; Lorite, Pedro; López-Casado, Miguel Ángel; Mora, Pablo; Palomeque, Teresa; Torres, María IsabelThis study investigates the role of immune checkpoint molecules in celiac disease (CD), focusing on the CD200/CD200R pathway and Elafin expression and their influence on immune regulation. Limited research exists on these molecules in CD, making these findings significant for understanding its clinical aspects. The results revealed elevated CD200 and CD200R levels and reduced PI3 expression in CD patients compared to healthy controls. CD200 expression, regulated by IFN-gamma, interacts with CD200R to stimulate Th1 and Th17 cytokine production. Specific SNPs, such as rs1733103 and rs41282752, were associated with CD, implicating genetic predisposition. The dysregulation of immune checkpoints in CD contributes to inflammation and autoimmunity, suggesting that therapies targeting these pathways could restore immune balance.Ítem Extracellular Vesicles: Advanced Tools for Disease Diagnosis, Monitoring, and Therapies(MDPI, 2024-12) Lorite, Pedro; Domínguez, Jorge Nicolas; Palomeque, Teresa; Torres, María IsabelExtracellular vesicles (EVs) are membrane-bound structures released by cells into their surrounding environment, facilitating intercellular communication through the transfer of proteins, lipids, and nucleic acids. These vesicles can be found in various body fluids, including blood, urine, saliva, and breast milk, between others. Due to their complexity, standardized methodologies are essential for isolating EVs with consistency and purity, enabling their application in diagnostics, therapy, and research. These EVs hold significant promise in advancing disease diagnosis and treatment, particularly in personalized and precision medicine. This review provides an updated overview of EVs, focusing on their functions, diagnostic and therapeutic applications, and the challenges in their clinical translation. Practical strategies to address these challenges are also discussed, aiming to bridge the gap between EV research and its implementation in healthcareÍtem Significance of PD1 Alternative Splicing in Celiac Disease as a Novel Source for Diagnostic and Therapeutic Target(2021-06) Ponce-de-León, Candelaria; Lorite, Pedro; López-Casado, Miguel Ángel; Barro, Francisco; Palomeque, Teresa; Torres-López, María IsabelThis study examines the role of the PD-1/PD-L1 pathway in celiac disease (CD), focusing on its involvement in immune regulation and the impact of altered mRNA splicing on identifying new diagnostic, prognostic, and therapeutic targets. The findings revealed an overexpression of the sPD-1 protein and the PD-1Dex3 transcript in CD. Three novel spliced isoforms were identified: two producing truncated proteins and one encoding a soluble PD-1 variant (sPD-1) due to a loss of exon 3 and part of exon 2. These results suggest that dietary gluten influences cell homeostasis by modulating pre-mRNA splicing of key regulatory proteins, serving as an adaptive response to dietary changes.Ítem Stimulatory Response of Celiac Disease Peripheral Blood Mononuclear Cells Induced by RNAi Wheat Lines Differing in Grain Protein Composition(MDPI, 2019-12) Sánchez-León, Susana; Giménez-Alvear, María José; Comino-Montilla, Isabel; Sousa, Carolina; López-Casado, Miguel Ángel; Torres-López, María Isabel; Barro, FranciscoGluten proteins in wheat are essential for bread-making but are also responsible for triggering gastrointestinal disorders such as celiac disease (CD), which affects approximately 1% of the Western population. Currently, the only effective treatment is a strict gluten-free diet. This study explores the use of RNA interference (RNAi) to reduce the expression of genes encoding immunogenic gluten proteins, offering a potential solution for developing CD-compatible cereals. These findings highlight the potential of RNAi technology to produce wheat varieties that are less immunogenic for CD patients while retaining some functional properties.