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Title: Xylitol production by Debaryomyces hansenii and Candida guilliermondii from rapeseed straw hemicellulosic hydrolysate
Authors: López-Linares, Juan Carlos
Romero, Inmaculada
Cara, Cristóbal
Castro, Eulogio
Mussatto, Solange
Abstract: This study evaluated the possibility of using rapeseed straw hemicellulosic hydrolysate as a fermentation medium for xylitol production. Two yeast strains, namely Debaryomyces hansenii and Candida guilliermondii, were used for this bioconversion process and their performance to convert xylose into xylitol was compared. Additionally, different strategies were evaluated for the hydrolysate detoxification before its use as a fermentation medium. Assays in semi-defined media containing different combinations of sugars were also performed in order to verify the influence of hexose sugars on xylose metabolism by the yeasts. C. guilliermondii exhibited higher tolerance to the toxic compounds than D. hansenii. Not only the toxic compounds present in the hydrolysate affected the yeast´s performance to convert xylose into xylitol, but glucose also had a negative impact on the bioconversion process. It was not necessary to completely eliminate the toxic compounds to obtain an efficient conversion of xylose into xylitol, mainly by C. guilliermondii.
Keywords: Rapeseed straw
Hemicellulosic hydrolysate
Semi-defined media
Issue Date: 2018
Publisher: Elsevier
Citation: Bioresource Technology 247 (2018) 736-743
Appears in Collections:DIQAM-Artículos

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