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Fully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacing

dc.contributor.authorMoreno-González, David
dc.contributor.authorHaselberg, Rob
dc.contributor.authorGámiz-Gracia, Laura
dc.contributor.authorGarcía-Campaña, Ana M.
dc.contributor.authorde Jong, Gerhardus J.
dc.contributor.authorSomsen, Govert W.
dc.date.accessioned2024-09-26T11:57:18Z
dc.date.available2024-09-26T11:57:18Z
dc.date.issued2017-11-17
dc.description.abstractThe on-line coupling of micellar electrokinetic chromatography and mass spectrometry (MEKC-MS) is often hampered by incompatibility problems leading to reduced separation performance and unfavorable limits of detection (LODs). Here we propose a new selective and highly sensitive MEKC-MS/MS method employing a sheathless porous-tip interface in combination with a micellar phase comprised of semi-volatile surfactant molecules. Carbamate pesticides (CRBs) were selected as representative model compounds being neutral toxic pollutants potentially present at trace levels in environmental water samples. A background electrolyte of 75 mM perfluorooctanoic acid adjusted to pH 9.0 with ammonium hydroxide allowed efficient separation of 15 CRBs and appeared fully compatible with electrospray ionization (ESI)-MS. Interfacing parameters, such as the distance between the capillary tip and mass-spectrometer inlet, ESI voltage, and dry gas temperature and flow were optimized in order to attain good spray stability and high analyte signal-to-noise ratios. For CRBs the LODs ranged from 0.2 to 3.9 ng L−1 (13 nL injected, i.e., 2% of capillary volume), representing an improvement for certain CRBs of more than 300-fold when compared with conventional sheath-liquid interfacing. Good linearity (R2 > 0.99) and satisfactory reproducibility were obtained for all CRBs with interday RSD values for peak area and migration time of 4.0–11.3% and below 1.5%, respectively. Analysis of spiked mineral water showed that the new MEKC-MS/MS method allows selective and quantitative determination of CRB concentrations below the maximum residue limit of 100 ng L−1 without the need for sample preconcentration.es_ES
dc.identifier.issn00219673es_ES
dc.identifier.other10.1016/j.chroma.2017.09.075es_ES
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0021967317314565es_ES
dc.identifier.urihttps://hdl.handle.net/10953/3247
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.ispartofJournal of Chromatography A Volume 1524, Pages 283 – 289es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subjectVolatile surfactantes_ES
dc.subjectCarbamateses_ES
dc.subjectMicellar electrokinetic chromatographyes_ES
dc.subjectMass spectrometryes_ES
dc.subjectSheathless interfacinges_ES
dc.subjectElectrospray ionizationes_ES
dc.titleFully Compatible and Ultra-Sensitive Micellar Electrokinetic Chromatography-Tandem Mass Spectrometry Using Sheathless Porous-Tip Interfacinges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones_ES

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