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Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

dc.contributor.authorBatista-García, Ramón
dc.contributor.authorPeidro-Guzmán, Heidy
dc.contributor.authorGonzález-Abradelo, Deborah
dc.contributor.authorFernández-López, Maikel
dc.contributor.authorDávila-Ramos, Sonia
dc.contributor.authorAranda-Ballesteros, Elizabet
dc.contributor.authorOrtega-García, Angélica
dc.contributor.authorLira, Verónica
dc.contributor.authorGunde-Cimerman, Nina
dc.contributor.authorRamírez-Pliego, Óscar
dc.contributor.authorSchnabel, Denhi
dc.contributor.authorJiménez-Gómez, Irina
dc.contributor.authorSánchez-Carbente, María
dc.contributor.authorFolch, Jorge Luis
dc.contributor.authorSánchez-Reyes, Ayixon
dc.contributor.authorKumar, Vaidyanathan Vinoth
dc.contributor.authorCabana, Hubert
dc.contributor.authorSantana, María Angélica
dc.contributor.authorMouriño-Pérez, Rosa Reyna
dc.contributor.authorAréchiga, Elva
dc.date.accessioned2025-01-30T07:22:35Z
dc.date.available2025-01-30T07:22:35Z
dc.date.issued2021
dc.description.abstractPolycyclic aromatic hydrocarbons (PAHs) are among the most persistent xenobiotic compounds, with high toxicity effects. Mycoremediation with halophilic Aspergillus sydowii was used for their removal from a hypersaline medium (1 M NaCl). A. sydowii metabolized PAHs as sole carbon sources, resulting in the removal of up to 90% for both PAHs [benzo [a] pyrene (BaP) and phenanthrene (Phe)] after 10 days. Elimination of Phe and BaP was almost exclusively due to biotransformation and not adsorption by dead mycelium and did not correlate with the activity of lignin modifying enzymes (LME). Transcriptomes of A. sydowii grown on PAHs, or on glucose as control, both at hypersaline conditions, revealed 170 upregulated and 76 downregulated genes. Upregulated genes were related to starvation, cell wall remodelling, degradation and metabolism of xenobiotics, DNA/RNA metabolism, energy generation, signalling and general stress responses. Changes of LME expression levels were not detected, while the chloroperoxidase gene, possibly related to detoxification processes in fungi, was strongly upregulated. We propose that two parallel metabolic pathways (mitochondrial and cytosolic) are involved in degradation and detoxification of PAHs in A. sydowii resulting in intracellular oxidation of PAHs. To the best of our knowledge, this is the most comprehensive transcriptomic analysis on fungal degradation of PAHs.
dc.identifier.other10.1111/1462-2920.15166es_ES
dc.identifier.urihttps://hdl.handle.net/10953/4544
dc.language.isoenges_ES
dc.publisherWileyes_ES
dc.relation.ispartofEnvironmental Microbiology (2021) 23(7), 3435–3459es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.titleTranscriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditionses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES

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