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A novel TctA citrate transporter from an activated sludge metagenome: Structural and mechanistic predictions for the TTT family

dc.contributor.authorBatista-García, Ramón
dc.contributor.authorSánchez-Reyes, Ayixon
dc.contributor.authorMillán, César
dc.contributor.authorGonzález-Zuñiga, Víctor Manuel
dc.contributor.authorFolch, Jorge Luis
dc.contributor.authorJuárez-Ramírez, Soledad
dc.date.accessioned2025-01-30T07:21:34Z
dc.date.available2025-01-30T07:21:34Z
dc.date.issued2014
dc.description.abstractWe isolated a putative citrate transporter of the tripartite tricarboxylate transporter (TTT) class from a metagenomic library of activated sludge from a sewage treatment plant. The transporter, dubbed TctA_ar, shares ~50% sequence identity with TctA of Comamonas testosteroni (TctA_ct) and other b-Proteobacteria, and contains two 20-amino acid repeat signature sequences, considered a hallmark of this particular transporter class. The structures for both TctA_ar and TctA_ct were modeled with I-TASSER and two possible structures for this transporter family were proposed. Docking assays with citrate resulted in the corresponding sets of proposed critical residues for function. These models suggest functions for the 20-amino acid repeats in the context of the two different architectures. This constitutes the first attempt at structure modeling of the TTT family, to the best of our knowledge, and could aid functional understanding of this little-studied family.
dc.identifier.other10.1002/prot.24529es_ES
dc.identifier.urihttps://hdl.handle.net/10953/4534
dc.language.isoenges_ES
dc.relation.ispartofProteins 2014; 00:000–000es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectMetagenomices_ES
dc.subjectTctA
dc.subjectMolecular modeling
dc.subjectDocking
dc.subjectGene phylogeny
dc.subjectCitrate
dc.titleA novel TctA citrate transporter from an activated sludge metagenome: Structural and mechanistic predictions for the TTT familyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES

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