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Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions

dc.contributor.authorBatista-García, Ramón
dc.contributor.authorMartínez-Ávila, Liliana
dc.contributor.authorPeidro-Guzmán, Heidy
dc.contributor.authorPérez-Llano, Yordanis
dc.contributor.authorMoreno-Perlin, Tonatiuh
dc.contributor.authorSánchez-Reyes, Ayixon
dc.contributor.authorFernández-Silva, Arline
dc.contributor.authorFolch, Jorge Luis
dc.contributor.authorCabana, Hubert
dc.contributor.authorGunde-Cimerman, Nina
dc.contributor.authorÁngeles, Gabriela
dc.contributor.authorAranda-Ballesteros, Elizabet
dc.date.accessioned2025-01-30T07:22:03Z
dc.date.available2025-01-30T07:22:03Z
dc.date.issued2021
dc.description.abstractPolyaromatic phenanthrene (Phe) and benzo[a]pyrene (BaP) are highly toxic, mutagenic, and carcinogenic contaminants widely dispersed in nature, including saline environments. Polyextremotolerant Rhodotorula mucilaginosa EXF-1630, isolated from Arctic sea ice, was grown on a huge concentration range -10 to 500 ppm- of Phe and BaP as sole carbon sources at hypersaline conditions (1 M NaCl). Selected polycyclic aromatic hydrocarbons (PAHs) supported growth as well as glucose, even at high PAH concentrations. Initially, up to 40% of Phe and BaP were adsorbed, followed by biodegradation, resulting in 80% removal in 10 days. While extracellular laccase, peroxidase, and un-specific peroxygenase activities were not detected, NADPH-cytochrome c reductase activity peaked at 4 days. The successful removal of PAHs and the absence of toxic metabolites were confirmed by toxicological tests on moss Physcomitrium patens, bacterium Aliivibrio fischeri, human erythrocytes, and pulmonary epithelial cells (A549). Metabolic profiles were determined at the midpoint of the biodegradation exponential phase, with added Phe and BaP (100 ppm) and 1 M NaCl. Different hydroxylated products were found in the culture medium, while the conjugative metabolite 1-phenanthryl-b-D glucopyranose was detected in the medium and in the cells. Transcriptome analysis resulted in 870 upregulated and 2,288 downregulated transcripts on PAHs, in comparison to glucose. Genomic mining of 61 available yeast genomes showed a widespread distribution of 31 xenobiotic degradation pathways in different yeast lineages. Two distributions with similar metabolic capacities included black yeasts and mainly members of the Sporidiobolaceae family (including EXF-1630), respectively. This is the first work describing a metabolic profile and transcriptomic analysis of PAH degradation by yeast.
dc.identifier.other10.1016/j.envpol.2020.116358es_ES
dc.identifier.urihttps://hdl.handle.net/10953/4539
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.ispartofEnvironmental Pollution 271 (2021) 116358es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectPolycyclic aromatic hydrocarbons es_ES
dc.subjectTranscriptomic
dc.subjectPAH metabolites
dc.subjectBioremediation
dc.subjectHalotolerant yeasts
dc.subjectBenzo[a]pyrene
dc.subjectPhenanthrene
dc.subjectRhodotorula mucilaginosa
dc.titleTracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditionses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersiones_ES

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