Microfluidic water-assisted trap focusing method for ultra-large volume injection in reversed-phase nano-liquid chromatography coupled to electron ionization tandem-mass spectrometry
dc.contributor.author | Termopoli, Veronica | |
dc.contributor.author | Famiglini, Giorgio | |
dc.contributor.author | Vocale, Palma | |
dc.contributor.author | Morini, Gian Luca | |
dc.contributor.author | Palma, Pierangela | |
dc.contributor.author | Rocío-Bautista, Priscilla | |
dc.contributor.author | Mansoor, Saeed | |
dc.contributor.author | Perry, Simon | |
dc.contributor.author | Cappiello, Achille | |
dc.date.accessioned | 2025-01-31T00:18:21Z | |
dc.date.available | 2025-01-31T00:18:21Z | |
dc.date.issued | 2020-09 | |
dc.description.abstract | Herein we present an efficient, column-switching method that relies on a custom-made T-union passive diffusion micromixer to assist water dilution and promote trap solute focusing of a high sample volume dissolved in pure organic solvent using a 0.075 mm i.d. nano-LC column. This method allows injecting 20 μL (or higher) of sample volume, speeding up the analysis time, with a 400-fold increase of the limits of quantitation for selected compounds. Five pesticides in different media were used as model compounds, and the analyses were carried out with a triple quadrupole mass spectrometer equipped with a Liquid Electron Ionization (LEI) LC-MS interface working in multiple reaction monitoring (MRM) mode. The system microfluidics were investigated using COMSOL modeling software. Robustness of the entire system was evaluated using a post-extraction addition soil extracts with limits of detection values spanning from 0.10 to 0.45 µg/L. Reproducible results in terms of peak area, peak shape, and retention times were achieved in soil matrix. Repeatability test on peak area variations were lower than 10%. | es_ES |
dc.identifier.issn | 0021-9673 | es_ES |
dc.identifier.other | doi.org/10.1016/j.chroma.2020.461421 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10953/4599 | |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | CC0 1.0 Universal | * |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/publicdomain/zero/1.0/ | * |
dc.subject | Ultra-large-volume injection | es_ES |
dc.subject | Nano-LC | es_ES |
dc.subject | Liquid electron ionization | es_ES |
dc.subject | mass spectrometry | es_ES |
dc.subject | Pesticides | es_ES |
dc.subject | Microfluidics | es_ES |
dc.title | Microfluidic water-assisted trap focusing method for ultra-large volume injection in reversed-phase nano-liquid chromatography coupled to electron ionization tandem-mass spectrometry | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.version | info:eu-repo/semantics/draft | es_ES |
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