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Microfluidic water-assisted trap focusing method for ultra-large volume injection in reversed-phase nano-liquid chromatography coupled to electron ionization tandem-mass spectrometry

dc.contributor.authorTermopoli, Veronica
dc.contributor.authorFamiglini, Giorgio
dc.contributor.authorVocale, Palma
dc.contributor.authorMorini, Gian Luca
dc.contributor.authorPalma, Pierangela
dc.contributor.authorRocío-Bautista, Priscilla
dc.contributor.authorMansoor, Saeed
dc.contributor.authorPerry, Simon
dc.contributor.authorCappiello, Achille
dc.date.accessioned2025-01-31T00:18:21Z
dc.date.available2025-01-31T00:18:21Z
dc.date.issued2020-09
dc.description.abstractHerein we present an efficient, column-switching method that relies on a custom-made T-union passive diffusion micromixer to assist water dilution and promote trap solute focusing of a high sample volume dissolved in pure organic solvent using a 0.075 mm i.d. nano-LC column. This method allows injecting 20 μL (or higher) of sample volume, speeding up the analysis time, with a 400-fold increase of the limits of quantitation for selected compounds. Five pesticides in different media were used as model compounds, and the analyses were carried out with a triple quadrupole mass spectrometer equipped with a Liquid Electron Ionization (LEI) LC-MS interface working in multiple reaction monitoring (MRM) mode. The system microfluidics were investigated using COMSOL modeling software. Robustness of the entire system was evaluated using a post-extraction addition soil extracts with limits of detection values spanning from 0.10 to 0.45 µg/L. Reproducible results in terms of peak area, peak shape, and retention times were achieved in soil matrix. Repeatability test on peak area variations were lower than 10%.es_ES
dc.identifier.issn0021-9673es_ES
dc.identifier.otherdoi.org/10.1016/j.chroma.2020.461421es_ES
dc.identifier.urihttps://hdl.handle.net/10953/4599
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsCC0 1.0 Universal*
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.subjectUltra-large-volume injectiones_ES
dc.subjectNano-LCes_ES
dc.subjectLiquid electron ionizationes_ES
dc.subjectmass spectrometryes_ES
dc.subjectPesticideses_ES
dc.subjectMicrofluidicses_ES
dc.titleMicrofluidic water-assisted trap focusing method for ultra-large volume injection in reversed-phase nano-liquid chromatography coupled to electron ionization tandem-mass spectrometryes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.type.versioninfo:eu-repo/semantics/draftes_ES

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