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Ephedra alata Subsp. Alenda as a novel source of bioactive phytochemicals: Characterization based on the mass spectrometry and profiling of antioxidant and anti-Inflammatory properties.

dc.contributor.authorMufti, Afoua
dc.contributor.authorContreras, María del Mar
dc.contributor.authorGómez-Cruz , Irene
dc.contributor.authorAlshamrani, Abdullah
dc.contributor.authorNahdi, Saber
dc.contributor.authorMansour, Lamjed
dc.contributor.authorAlwasel, Salah
dc.contributor.authorHalim Harrath, Abdel
dc.contributor.authorTlili, Nizar
dc.date.accessioned2025-02-12T12:26:26Z
dc.date.available2025-02-12T12:26:26Z
dc.date.issued2023-01-23
dc.description.abstractThe aim of the present study was to examine, for the first time, the phytochemical content of Ephedra alata pulp extract (EAP) and explore its antioxidant and anti-inflammatory capacities. High-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF/MS) was used for phytochemical analysis and three in vitro antioxidant assays together with three in vitro anti-inflammatory tests were used for the assessment of biological activity. The HPLC-ESI-QTOF/MS analysis revealed the presence of 42 metabolites, including flavonoids, sphingolipides, fatty acids, ephedrine derivatives, and amino acid derivatives. In vitro findings revealed that EAP has interesting 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, and ferrous ion chelating capacities (IC50 values were 0.57 mg/mL, 0.55 mg/mL, and 0.51 mg/mL for DPPH, superoxide radical, and ferrous ion, respectively). Furthermore, EAP showed a noticeable anti-inflammatory ability by inhibiting the two cyclooxygenase isoforms, COX-1 and COX-2 (IC50 of 59.1 and 58.8 µg/mL for COX-1 and COX-2, respectively), preventing protein denaturation (IC50 = 0.51 mg/mL), and protecting membrane stabilization (IC50 = 0.53 mg/mL). The results highlighted the use of Ephedra alata pulp as a potential source of natural compounds with therapeutic effects for the management of inflammatory disorders.
dc.description.sponsorshipThe Ministry of Higher Education and Scientific Research, Tunisia (Project number: 19P EJC06-04). The King Saud University, Riyadh, Saudi Arabia, for their support (Project number RSP-2021/17).
dc.identifier.citationMufti A, Contreras MDM, Gómez-Cruz I, Alshamrani A, Nahdi S, Mansour L, Alwasel S, Harrath AH, Tlili N. Ephedra alata Subsp. Alenda as a Novel Source of Bioactive Phytochemicals: Characterization Based on the Mass Spectrometry and Profiling of Antioxidant and Anti-Inflammatory Properties. Life (Basel). 2023 Jan 23;13(2):323. doi: 10.3390/life13020323
dc.identifier.issn2075-1729
dc.identifier.other10.3390/life13020323
dc.identifier.urihttps://doi.org/10.3390/life13020323
dc.identifier.urihttps://hdl.handle.net/10953/4678
dc.language.isoeng
dc.publisherMDPI
dc.relation.ispartofLife (Basel) [2023];[13(2)]:[323]
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spainen
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectEphedra alata
dc.subjectHigh-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC–ESI–QTOF/MS)
dc.subjectAnti-inflammatory
dc.subjectCOX1 and COX2
dc.subjectAntioxidants
dc.titleEphedra alata Subsp. Alenda as a novel source of bioactive phytochemicals: Characterization based on the mass spectrometry and profiling of antioxidant and anti-Inflammatory properties.
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion

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