Ephedra alata Subsp. Alenda as a novel source of bioactive phytochemicals: Characterization based on the mass spectrometry and profiling of antioxidant and anti-Inflammatory properties.
dc.contributor.author | Mufti, Afoua | |
dc.contributor.author | Contreras, María del Mar | |
dc.contributor.author | Gómez-Cruz , Irene | |
dc.contributor.author | Alshamrani, Abdullah | |
dc.contributor.author | Nahdi, Saber | |
dc.contributor.author | Mansour, Lamjed | |
dc.contributor.author | Alwasel, Salah | |
dc.contributor.author | Halim Harrath, Abdel | |
dc.contributor.author | Tlili, Nizar | |
dc.date.accessioned | 2025-02-12T12:26:26Z | |
dc.date.available | 2025-02-12T12:26:26Z | |
dc.date.issued | 2023-01-23 | |
dc.description.abstract | The aim of the present study was to examine, for the first time, the phytochemical content of Ephedra alata pulp extract (EAP) and explore its antioxidant and anti-inflammatory capacities. High-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF/MS) was used for phytochemical analysis and three in vitro antioxidant assays together with three in vitro anti-inflammatory tests were used for the assessment of biological activity. The HPLC-ESI-QTOF/MS analysis revealed the presence of 42 metabolites, including flavonoids, sphingolipides, fatty acids, ephedrine derivatives, and amino acid derivatives. In vitro findings revealed that EAP has interesting 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, and ferrous ion chelating capacities (IC50 values were 0.57 mg/mL, 0.55 mg/mL, and 0.51 mg/mL for DPPH, superoxide radical, and ferrous ion, respectively). Furthermore, EAP showed a noticeable anti-inflammatory ability by inhibiting the two cyclooxygenase isoforms, COX-1 and COX-2 (IC50 of 59.1 and 58.8 µg/mL for COX-1 and COX-2, respectively), preventing protein denaturation (IC50 = 0.51 mg/mL), and protecting membrane stabilization (IC50 = 0.53 mg/mL). The results highlighted the use of Ephedra alata pulp as a potential source of natural compounds with therapeutic effects for the management of inflammatory disorders. | |
dc.description.sponsorship | The Ministry of Higher Education and Scientific Research, Tunisia (Project number: 19P EJC06-04). The King Saud University, Riyadh, Saudi Arabia, for their support (Project number RSP-2021/17). | |
dc.identifier.citation | Mufti A, Contreras MDM, Gómez-Cruz I, Alshamrani A, Nahdi S, Mansour L, Alwasel S, Harrath AH, Tlili N. Ephedra alata Subsp. Alenda as a Novel Source of Bioactive Phytochemicals: Characterization Based on the Mass Spectrometry and Profiling of Antioxidant and Anti-Inflammatory Properties. Life (Basel). 2023 Jan 23;13(2):323. doi: 10.3390/life13020323 | |
dc.identifier.issn | 2075-1729 | |
dc.identifier.other | 10.3390/life13020323 | |
dc.identifier.uri | https://doi.org/10.3390/life13020323 | |
dc.identifier.uri | https://hdl.handle.net/10953/4678 | |
dc.language.iso | eng | |
dc.publisher | MDPI | |
dc.relation.ispartof | Life (Basel) [2023];[13(2)]:[323] | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain | en |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | |
dc.subject | Ephedra alata | |
dc.subject | High-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC–ESI–QTOF/MS) | |
dc.subject | Anti-inflammatory | |
dc.subject | COX1 and COX2 | |
dc.subject | Antioxidants | |
dc.title | Ephedra alata Subsp. Alenda as a novel source of bioactive phytochemicals: Characterization based on the mass spectrometry and profiling of antioxidant and anti-Inflammatory properties. | |
dc.type | info:eu-repo/semantics/article | |
dc.type.version | info:eu-repo/semantics/publishedVersion |
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