Examinando por Autor "Romero-Pulido, Inmaculada"
Mostrando 1 - 2 de 2
- Resultados por página
- Opciones de ordenación
Ítem Hepatopreventive properties of hydroxytyrosol and mannitol-rich extracts obtained from exhausted olive pomace using green extraction methods(Royal Society of Chemistry, 2022-12-25) Contreras, María del Mar; Gómez -Cruz, Irene; Feriani, Anouar; Alwasel, Saleh; Harrath, Abdel Halim; Romero-Pulido, Inmaculada; Castro-Galiano, Eulogio; Tlili, NizarExhausted olive pomace (EOP) is produced in olive-pomace oil extractors as a by-product. However, the obtention of bioactive compounds from EOP can reinsert it into the economy as a new bioresource before applying other exploitation ways. The objective of the present study was to investigate the phytochemical differences between aqueous and aqueous acetonic extracts from EOP (AE-EOP and AAE-EOP, respectively) obtained by hydrothermal and ultrasound-assisted extraction, respectively. The in vitro antioxidant activities and the in vivo hepatopreventive potential were evaluated. Using RP-HPLC–ESI–QTOF– MS, the chemical profile revealed that AE-EOP and AAE-EOP showed similar qualitative profiles, with some quantitative differences. Hydroxytyrosol and mannitol were the major compounds of the extracts. The investigation of antioxidant properties in vitro highlighted that AE-EOP was slightly more efficient in scavenging DPPH, ABTS, superoxide, and hydrogen peroxide radicals, when compared to AAE-EOP. Additionally, AE-EOP and AAE-EOP showed dose-dependent suppressive effects on pancreatic lipase activity. In vivo studies showed that AE-EOP and AAE-EOP presented interesting hepatopreventive capacities against CCl4 induced liver injury, as evidenced by (i) the preventive effects against DNA damage, (ii) the normalised hepatic biomarker parameters (ALT, AST, GGT, and LDH) and (iii) the normalised lipid profile (LDL-C, TC, TG, and HDL-C) through diminishing their levels, (iv) which was supported by Oil Red O analysis. Furthermore, AE-EOP and AAE-EOP reduced the oxidative stress in liver tissue by inhibiting lipid peroxidation together with the enhancement of the hepatic antioxidant activities (CAT, SOD and GPx) and GSH content. Additionally, AE-EOP and AAE-EOP exhibited an antifibrotic effect, which was clearly demonstrated by the histopathological examination using Picrosirius red staining. The obtained results support the use of EOP extracts in industries without further purification as antioxidants and against free radical induced damage.Ítem High Solids Loading Pretreatment of Olive Tree Pruning with Dilute Phosphoric Acid for Bioethanol Production by Escherichia coli(American Chemical Society, 2015-02-26) Martínez-Patiño, José Carlos; Romero-García, Juan Miguel; Ruiz-Ramos, Encarnación; Oliva, José Miguel; Álvarez-Vaquerizo, Cristina; Romero-Pulido, Inmaculada; Negro, María José; Castro-Galiano, EulogioThis paper deals with a new approach for using olive tree pruning biomass as raw material for ethanol and other chemical production. This process includes a water extraction step, followed by a high solids loading dilute phosphoric acid pretreatment and an ethanologenic Escherichia coli fermentation for the conversion of all of the sugars released. Results show that the operational conditions leading to the maximal hemicelullosic sugar recovery in the liquid fraction (near 70%) are 170 °C and 0.5% (w/v) phosphoric acid concentration. The fermentation of the mixed sugars found in the detoxified prehydrolysates by ethanologenic E. coli MS04 led to an ethanol concentration of 23 g/L and an ethanol yield of 0.46 g of ethanol/g of sugars consumed. When the ethanol production from the pretreated solids after enzymatic hydrolysis is also taken into account, 13.2 g of ethanol/100 g of original material was obtained. The production of other interesting compounds is also considered.