Examinando por Autor "Ovelleiro, David"
Mostrando 1 - 3 de 3
- Resultados por página
- Opciones de ordenación
Ítem APPLIED QUANTITATIVE PROTEOMICS ANALYSIS(Jaén : Universidad de Jaén, 2020-04-03) Ovelleiro, David; Peinado-Herreros, María Ángeles; Blanco-Ruiz, Santos; Universidad de Jaén. Departamento de Biología ExperimentalEn esta tesis se ha aplicado el estado del arte en análisis cuantitativo en proteómica. Los datos analizados en este trabajo, provenientes de tres proyectos distintos, fueron obtenidos usando tres de las técnicas más utilizadas en proteómica: cuantificación label-free, marcaje isobárico y SWATH. Los resultados obtenidos en los diferentes proyectos son también interpretados mediante múltiples herramientas bioinformáticas. La cuantificación label-free es utilizada aquí para obtener la combinación óptima de software y parámetros usando un conjunto de datos públicos. El marcaje isobárico, usando TMT, se emplea en el estudio de los diferentes perfiles de expresión proteica, obtenidos con dos modelos de hipoxia de diferente severidad en cerebros de rata. La técnica SWATH se busca en la búsqueda de biomarcadores de síndorme de ovario poliquístico en plasma. Por último, los elementos necesarios para la implantación de una plataforma de análisis proteómica , en términos de software y hardware, se describen en forma detallada. In this thesis, the state of the art in quantitative proteomics analysis has been applied. The data analyzed in this work, coming from three different projects, were acquired using three of the most used techniques in proteomics: label-free, isobaric labeling and SWATH. The results obtained in the different projects are also interpreted using multiple bioinformatics tools. The label-free quantization is used here to asses the optimal combination of software and parameters using a public data set. Isobaric labeling, using TMT, is employed to study the different profiles in protein expression when two hypoxic models, with different severity, are applied in rat brains. The SWATH technique is used in the search of biomarkers for polycystic ovary syndrome in plasma. Finally, the elements required for setting up a platform for proteomics analysis, both in terms of hardware and software, are comprehensively described.Ítem Biological Implications of a Stroke Therapy Based in Neuroglobin Hyaluronate Nanoparticles. Neuroprotective Role and Molecular Bases(MDPI, 2022) Peinado-Herreros, María Ángeles; Ovelleiro, David; del-Moral, María Luisa; Hernández-Cobo, Raquel; Martínez-Lara, Esther; Siles, Eva; Pedrajas, José Rafael; García-Martín, María Luisa; Caro, Carlos; Peralta, Sebastián; Morales-Hernández, María Encarnación; Ruiz-Martínez, María Adolfina; Blanco-Ruiz, SantosExogenous neuroprotective protein neuroglobin (Ngb) cannot cross the blood–brain barrier. To overcome this difficulty, we synthesized hyaluronate nanoparticles (NPs), able to deliver Ngb into the brain in an animal model of stroke (MCAO). These NPs effectively reached neurons, and were microscopically identified after 24 h of reperfusion. Compared to MCAO non-treated animals, those treated with Ngb-NPs showed survival rates up to 50% higher, and better neurological scores. Tissue damage improved with the treatment, but no changes in the infarct volume or in the oxidative/nitrosative values were detected. A proteomics approach (p-value < 0.02; fold change = 0.05) in the infarcted areas showed a total of 219 proteins that significantly changed their expression after stroke and treatment with Ngb-NPs. Of special interest, are proteins such as FBXO7 and NTRK2, which were downexpressed in stroke, but overexpressed after treatment with Ngb-NPs; and ATX2L, which was overexpressed only under the effect of Ngb. Interestingly, the proteins affected by the treatment with Ngb were involved in mitochondrial function and cell death, endocytosis, protein metabolism, cytoskeletal remodeling, or synaptic function, and in regenerative processes, such as dendritogenesis, neuritogenesis, or sinaptogenesis. Consequently, our pharmaceutical preparation may open new therapeutic scopes for stroke and possibly for other neurodegenerative pathologies.Ítem Comparative proteomic study of early hypoxic response in the cerebral cortex of rats submitted to two different hypoxic models(Wiley - V C H Verlag GmbH, 2017) Ovelleiro, David; Blanco-Ruiz, Santos; Hernández-Cobo, Raquel; Peinado-Herreros, María ÁngelesPurpose: The present study analyses and compares the cortical brain proteomic profiles of two different models of cerebral hypoxic insult in rats (HH: hypobaric hypoxia and HHI: ischemia followed by hypobaric hypoxia) in an attempt to describe the alterations of the early molecular hypoxic adaptive response underlying each one. Experimental Design: A quantitative proteomic profile of left-brain cortices of rats under HH, HHI, and control conditions was determined using isobaric labeling (Tandem Mass TagTM) on the protein extracts from pools of five individuals. Data are available via ProteomeXchange with identifier PXD004091. Results: Altogether, 339 proteins were confidently quantified, 99 of them showing significant variations in the hypoxic conditions with respect to the control. The HHI model presents a global effect of protein downregulation while HH produces an overall increase of the protein levels. While HH mainly affecting oxidative and energetic metabolism, HHI also interferes with synaptic transmission, neurotransmitter secretion, substantia nigra development, and triggers apoptosis through mitochondrial pathway. Conclusions and Clinical Relevance: The findings obtained show an overview of protein alterations under two hypoxic models of different aetiology and provide a basis for more detailed studies in order to unravel new specific mechanisms and therapies for hypoxic pathologies.