Examinando por Autor "Mora-Ruiz, Pablo"
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Ítem Comparative cytogenetics of Lachnaia species (Coleoptera: Chrysomelidae) reveals a novel telomeric motif (TTTGG) in insects(Springer, 2025-03-27) Rico-Porras, Jose Manuel; Mora-Ruiz, Pablo; Palomeque, Teresa; Cabral-de-Mello, Diogo; Lorite, PedroCytogenetic analyses of Lachnaia hirta, Lachnaia tristigma, and Lachnaia vicina have revealed that all species show a similar karyotype with 2n=24 and a meioformula of 11+Xy+. Despite this, their sex chromosomes show certain diferences in morphology. All species exhibit pericentromeric heterochromatin on all autosomes and X chromosomes, with the Y chromosome being heterochromatic. The X and Y chromosomes carry the nucleolar organizer regions in the three species, althoug L. vicina also has them in one autosomal pair. No clear hybridization signals were obtained using a probe with the TTAGG repeat, which is the ancestral DNA motif of telomeres in insects. The genomes of the three species have been sequenced. The obtained data have been used for the identifcation of telomeric motifs through bioinformatics analyses, including the Telomeric Repeats Identifcation Pipeline, a bioinformatics tool for identifying telomeric repeat motifs. According to the data, the TTTGG sequence is suggested as the telomeric repeat in these species, a fnding confrmed by fuorescence in situ hybridization. The TTTGG motif constitutes a new telomeric repeat motif in Coleoptera. This new motif has been found at the chromosome ends of another beetle species, the oak borer Platypus cylindrus (Coleoptera, Curculionidae), and in a wasp species, Oxytorus armatus (Hymenoptera, Ichneumonoidea), whose genomes are assembled at the chromosome level. The detection of this telomeric repeat in two families of beetles and in a hymenopteran species suggests that this new telomeric motif has independently emerged in evolutionarily distant groups of insects.Ítem The first insight into Acanthocephalus (Palaeacanthocephala) satellitome: species-specific satellites as potential cytogenetic markers(Springer Nature, 2025-01-23) Marková, Anna; Orosová, Martina; Mora-Ruiz, Pablo; Benovics, Michal; Lorite, PedroAcanthocephalan parasites are often overlooked in many areas of research, and satellitome and cytogenetic analyzes are no exception. The species of the genus Acanthocephalus are known for their very small chromosomes with ambiguous morphology, which makes karyotyping difficult. In this study, we performed the first satellitome analysis of three Acanthocephalus species to identify species- and chromosome-specific satellites that could serve as cytogenetic markers. RepeatExplorer2 revealed a remarkably high number of species-specific repeats, with a predominance of satellite DNAs, alongside variations in repetitive content between sexes. Five satellites in A. anguillae, two in A. lucii and six in A. ranae were successfully mapped to chromosomes using FISH. Each satellite showed a clustered hybridization signal at specific chromosomal locations, which allowed us to create a schematic representation of the distribution of satellites for each species. These newly identified satellites proved to be useful chromosomal markers for the accurate identification of homologous chromosome pairs. No FISH-positive signals were observed on the supernumerary chromosomes of A. anguillae and A. lucii, supporting the hypothesis that these chromosomes have recent origin.