Examinando por Autor "Molina-Ortega, Francisco Javier"
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Ítem Are patients with fibromyalgia in a prothrombotic state?(Sage Publications Inc, 2019) Molina-Ortega, Francisco Javier; del-Moral, María Luisa; la-Rubia, Mercedes; Blanco-Ruiz, Santos; Carmona-Martos, Ramón; Rus-Martínez, AlmaObjectives: The aim of this study was to investigate thrombosis-related parameters (blood coagulation parameters, platelet indices, red blood cell [RBC] count, and inflammatory markers) in patients with fibromyalgia (FM). Method: We carried out a case–control study with 35 women with FM and 12 age-matched healthy volunteers to analyze fibrinogen levels, prothrombin time, cephaline time, platelet count, platelet distribution width (PDW), mean platelet volume (MPV), RBC count, neutrophil-tolymphocyte ratio, and platelet-to-lymphocyte ratio (PLR). Results: The results showed significantly increased fibrinogen levels (p < .05), platelet count (p < .05), PDW (p ¼ .059), RBC count (p < .05), and PLR (p < .05) in women with FM versus the healthy volunteers. Prothrombin time (p < .05) and MPV (p < .05) were significantly lower in patients with FM than in the controls. Conclusions: Elevated platelet and RBC counts, PDW values, and fibrinogen levels as well as decreased prothrombin time are all indicative of a prothrombotic state in FM patients, which may be enhanced by an increased inflammatory tone. This prothrombotic state may increase the risk of thrombosis-related cardiovascular disease in patients with FM.Ítem Immunohistochemistry of neuronal nitric oxide synthase and protein nitration in the striatum of the aged rat(John Wiley & Sons, Inc., 2004) del-Moral, María Luisa; Esteban-Ruiz, Francisco José; Hernández-Cobo, Raquel; Blanco-Ruiz, Santos; Molina-Ortega, Francisco Javier; Martínez-Lara, Esther; Siles, Eva; Viedma, Gloria; Ruiz, Alharilla; Pedrosa-Raya, Juan Ángel; Peinado-Herreros, María ÁngelesTo ascertain the possible implications of the nitric oxide (NO● ) producing system in striatal senescence, and by using immunohistochemistry and image-processing approaches, we describe the presence of the enzyme nitric oxide synthase (NOS), the NADPH-diaphorase (NADPH-d) histochemical marker, and nitrotyrosine-derived complexes (N-Tyr) in the striatum of adult and aged rats. The results showed neuronal NOS immunoreactive (nNOS-IR) aspiny medium-sized neurons and nervous fibres in both age groups, with no variation in the percentage of immunoreactive area but a significant decrease in the intensity and in the number of somata with age, which were not related to the observed increase with age of the striatal bundles of the white matter. In addition, NADPH-d activity was detected in neurons with morphology similar to that of the nNOS-IR cells; a decrease in the percentage of area per field and in the number of cells, but an increase in the intensity of staining for the NADPH-d histochemical marker, were detected with age. The number of neuronal NADPH-d somata was higher than for the nNOS-IR ones in both age groups. Moreover, N-Tyr-IR complexes were observed in cells (neurons and glia) and fibres, with a significant increase in the percentage of the area of immunoreaction, related to the increase of white matter, but a decrease in intensity for the aged group. On the other hand, we did not detect the inducible isoform (iNOS) either in adult or in aged rats. Taken together, these results support the contention that NADPH-d staining is not such an unambiguous marker for nNOS, and that increased protein nitration may participate in striatal aging.Ítem Manual Therapy Techniques Versus Occlusal Splint Therapy for Temporomandibular Disorders: A Systematic Review with Meta-Analysis(MDPI, 2024-11-01) Villar-Aragón, Víctor; Obrero, Esteban; Lérida-Ortega, Miguel Ángel; López-Ruiz, María del Carmen; Rodríguez-Almagro, Daniel; Achalandabaso-Ochoa, Alexander; Molina-Ortega, Francisco Javier; Ibáñez-Vera, Alfonso JavierBackground: Manual therapy (MT) and occlusal splint therapy (OST) are the most conservative therapies applied on patients with temporomandibular disorders (TMDs). The aim was to compare the efficacy of MT vs. OST in improving pain, maximal mouth opening (MMO), disability, and health related-quality of life (hr-QoL) in these patients. Methods: According to PRISMA guidelines, a meta-analysis (CRD42022343915) was conducted including randomized controlled trials comparing the effectiveness of MT vs. OST in TMD patients, after searching in PubMed, PEDro, SCOPUS, and WOS up to March 2024. Methodological quality and risk of bias were assessed using the PEDro Scale. Cohen’s standardized mean difference (SMD) and its 95% confidence interval (95% CI) were the pooled effect measures calculated. Results: Nine studies, providing data from 426 patients, were included. Meta-analyses revealed that MT is more effective than OST in reducing disability (SMD = −0.81; 95% CI −1.1 to −0.54) and increasing MMO (SMD = 0.52; 95% CI 0.27 to 0.76) without differences for improving pain intensity and hr-QoL. Subgroup analyses revealed the major efficacy of OST in reducing pain in myogenic patients (SMD = 0.65; 95% CI 0.02 to 1.28). Conclusions: With caution, due to the low number of studies included, MT may be more effective than OST for improving disability and MMO in patients with TMDs.Ítem Study of the nitric oxide system in the rat cerebellum during aging(BioMed Central Ltd, 2010) Blanco-Ruiz, Santos; Molina-Ortega, Francisco Javier; Castro-Ortega, Lourdes; del-Moral, María Luisa; Hernández-Cobo, Raquel; Jiménez, Ana; Rus-Martínez, Alma; Martínez-Lara, Esther; Siles, Eva; Peinado-Herreros, María ÁngelesBackground: The cerebellum is the neural structure with the highest levels of nitric oxide, a neurotransmitter that has been proposed to play a key role in the brain aging, although knowledge concerning its contribution to cerebellar senescence is still unclear, due mainly to absence of integrative studies that jointly evaluate the main factors involved in its cell production and function. Consequently, in the present study, we investigate the expression, location, and activity of nitric oxide synthase isoenzymes; the protein nitration; and the production of nitric oxide in the cerebellum of adult and old rats. Results: Our results show no variation in the expression of nitric oxide synthase isoforms with aging, although, we have detected some changes in the cellular distribution pattern of the inducible isoform particularly in the cerebellar nuclei. There is also an increase in nitric oxide synthase activity, as well as greater protein-nitration levels, and maintenance of nitrogen oxides (NOx) levels in the senescent cerebellum. Conclusions: The nitric oxide/nitric oxide syntahses system suffers from a number of changes, mainly in the inducible nitric oxide synthase distribution and in overall nitric oxide synthases activity in the senescent cerebellum, which result in an increase of the protein nitration. These changes might be related to the oxidative damage detected with aging in the cerebellum.