Examinando por Autor "Boulaiz, Houria"
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Ítem Cellular extracts from post-mortem human cardiac tissue direct cardiomyogenic differentiation of human adipose tissue-derived stem cells(Elsevier, 2010) Perán, Macarena; Marchal, Juan Antonio; López-Ruiz, Elena; Jiménez-Navarro, Manuel; Boulaiz, Houria; Rodríguez-Serrano, Fernado; Carrillo-Delgado, Esmeralda; Sánchez-Espín, Gema; de-Teresa, Eduardo; Tosh, David; Aránega, AntoniaBackground aims. The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Methods. Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture. Results. After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fibers), immunofl uorescence detection of cardiac-specifi c markers (connexin-43, sarcomeric a-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription – polymerase chain reaction (cardiac myosin light chain 1, a -cardiac actin, cardiac troponin T and cardiac b -myosin). Conclusions. We have demonstrated for the fi rst time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease.Ítem Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes(Elsevier, 2010) Perán, Macarena; Marchal, Juan Antonio; López-Ruiz, Elena; Jiménez-Navarro, Manuel; Boulaiz, Houria; Rodríguez-Serrano, Fernando; Carrillo-Delgado, Esmeralda; Sánchez-Espín, Gema; de-Teresa, Eduardo; Tosh, David; Aránega, AntoniaBackground aims. The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Methods. Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture. Results. After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fi bers), immunofl uorescence detection of cardiac-specifi c markers (connexin-43, sarcomeric a-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription – polymerase chain reaction (cardiac myosin light chain 1, a cardiac actin, cardiac troponin T and cardiac b-myosin). Conclusions. We have demonstrated for the fi rst time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease.Ítem Papel de las células madre tumorales en la respuesta a nuevos fármacos selectivos frente a cáncer de colon y mama(Jaén : Universidad de Jaén, 2014) Ramírez-Rivera, Alberto; Marchal, Juan Antonio; Perán, Macarena; Boulaiz, Houria; Universidad de Jaén. Departamento de Ciencias de la Salud[ES] El cáncer es una de las enfermedades más importantes a nivel mundial. Las células madre cancerígenas (CSC) son clave en el proceso de tumorigénesis y en la aparición de quimio y radioresistencia, facilitando la aparición de recurrencia y metástasis. Actualmente hay un gran interés en desarrollar terapias selectivas frente a las células tumorales y las CSC. Nuestro objetivo ha sido seleccionar, de un conjunto de fármacos de nueva síntesis, el compuesto con más actividad antitumoral. Bozepinib ha mostrado una potente y selectiva actividad antiproliferativa frente a células tumorales y CSC. Además, actúa sobre vías metabólicas de la tumorigenesis y CSCs, poniendo de relieve su actividad anti cses. In vivo, Bozepinib no induce toxicidad aguda ni crónica a altas dosis y posee una potente actividad antitumoral. Estos resultados indican la eficacia de Bozepinib como agente farmacológico frente a CSCs pudiendo mejorar la respuesta de los pacientes con este tipo de patología.Ítem Ultrastructural and molecular analyzes of insulin-producing cells induced from human hepatoma cells(Elsevier, 2011) Perán, Macarena; Sánchez-Ferrero, Aitor; Tosh, David; Marchal, Juan Antonio; López-Ruiz, Elena; Álvarez-Aránega, Pablo; Boulaiz, Houria; Rodríguez-Serrano, Fernando; Aránega, AntoniaBackground aims. Diabetes type I is an autoimmune disease characterized by the destruction of pancreatic insulin-producing (beta-) cells and resulting in external insulin dependence for life. Islet transplantation represents a potential treatment for diabetes but there is currently a shortage of suitable organs donors. To augment the supply of donors, different strategies are required to provide a potential source of beta-cells. These sources include embryonic and adult stem cells as well as differentiated cell types. The main goal of this study was to induce the transdifferentiation (or conversion of one type cell to another) of human hepatoma cells (HepG2 cells) to insulin-expressing cells based on the exposure of HepG2 cells to an extract of rat insulinoma cells (RIN). Methods. HepG2 cells were fi rst transiently permeabilized with Streptolysin O and then exposed to a cell extract obtained from RIN cells. Following transient exposure to the RIN extract, the HepG2 cells were cultured for 3 weeks. Results. Acquisition of the insulin-producing cell phenotype was determined on the basis of (i) morphologic and (ii) ultrastructural observations, (iii) immunologic detection and (iv) reverse transcription (RT)-polymerase chain reaction (PCR) analysis. Conclusions. This study supports the use of cell extract as a feasible method for achieve transdifferentiation of hepatic cells to insulin-producing cells.