Begara-Morales, Juan CarlosChaki, MouniraSánchez-Calvo, BeatrizMata-Pérez, CapillaLeterrier, MarinaPalma, José ManuelBarroso-Albarracín, Juan BautistaCorpas, Francisco Javier2025-01-192025-01-192013-01-28Begara-Morales JC., Chaki M., Sanchez-Calvo B., Mata-Perez C., Leterrier M., Palma JM., Barroso JB., Corpas FJ. "Protein tyrosine nitration in pea roots during development and senescence"0022-095710.1093/jxb/ert006https://hdl.handle.net/10953/4120Protein tyrosine nitration is a post-translational modification mediated by reactive nitrogen species (RNS) that is associated with nitro-oxidative damage. No information about this process is available in relation to higher plants during development and senescence. Using pea plants at different developmental stages (ranging from 8 to 71 days), tyrosine nitration in the main organs (roots, stems, leaves, flowers, and fruits) was analysed using immunological and proteomic approaches. In the roots of 71-day-old senescent plants, nitroproteome analysis enabled the identification a total of 16 nitrotyrosine-immunopositive proteins. Among the proteins identified, NADP-isocitrate dehydrogenase (ICDH), an enzyme involved in the carbon and nitrogen metabolism, redox regulation, and responses to oxidative stress, was selected to evaluate the effect of nitration. NADP-ICDH activity fell by 75% during senescence. Analysis showed that peroxynitrite inhibits recombinant cytosolic NADP-ICDH activity through a process of nitration. Of the 12 tyrosines present in this enzyme, mass spectrometric analysis of nitrated recombinant cytosolic NADP-ICDH enabled this study to identify the Tyr392 as exclusively nitrated by peroxynitrite. The data as a whole reveal that protein tyrosine nitration is a nitric oxide-derived PTM prevalent throughout root development and intensifies during senescence.engNitrationNitric oxideNitrotyrosinePeroxynitritePlant developmentReactive nitrogen speciesSenescenceinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess