Grande-Burgos, María JoséLucas-López, RosarioAbriouel, HikmateValdivia, EvaBen Omar, NabilMaqueda, MercedesMartíneza-Cañamero, MagdalenaGálvez, Antonio2024-02-032024-02-032006-08-01MaJ. Grande, R. Lucas, H. Abriouel, E. Valdivia, N. Ben Omar, M. Maqueda, M. Martínez‐Cañamero, A. Gálvez, Inhibition of Bacillus licheniformis LMG 19409 from ropy cider by enterocin AS‐48, Journal of Applied Microbiology, Volume 101, Issue 2, 1 August 2006, Pages 422–428,ISSN 1364-5072https://doi.org/10.1111/j.1365-2672.2006.02942.xhttps://academic.oup.com/jambio/article/101/2/422/6718482https://hdl.handle.net/10953/1940This work was supported by the Spanish Ministry ofScience and Technology (research project AGL2001-3315-CO2-02). We also acknowledge the Research Plan ofthe Junta de Andalucı´a (research group AGR230) fromwhich M a J. Grande received a fellowship and theResearch Programme of the University of Jaén.Aims: To determine the activity of enterocin AS-48 against ropy-forming Bacil-lus licheniformis from cider.Methods and Results: Enterocin AS-48 was tested on B. licheniformis LMG19409 from ropy cider in MRS-G broth, fresh-made apple juice and in twocommercial apple ciders (A and B). Bacillus licheniformis was rapidly inacti-vated in MRS-G by 0Æ5 lg ml)1 AS-48 and in fresh-made apple juice by3 lg ml)1 . Concentration-dependent inactivation of this bacterium in twocommercial apple ciders (A and B) stored at 4, 15 and 30°C for 15 days wasalso demonstrated. Counts from heat-activated endospores in cider A plusAS-48 decreased very slowly. Application of combined treatments of heat(95°C) and enterocin AS-48 reduced the time required to achieved completeinactivation of intact spores in cider A to 4 min for 6 lg ml)1 and to 1 minfor 12 lg ml)1 . D and z values also decreased as the bacteriocin concentrationincreased.Conclusion: Enterocin AS-48 can inhibit ropy-forming B. licheniformis in applecider and increase the heat sensitivity of spores.Significance and Impact of the Study: Results from this study support thepotential use of enterocin AS-48 to control B. licheniformis in apple cider.engCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/Bacillus licheniformisBacteriocinCiderEnterocinRopinessinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess