Lozano-Velasco, EstefaníaVallejo-Pulido, DanielEsteban-Ruiz, Francisco JoséDoherty, ChrisHernández-Torres, FranciscoFranco, DiegoAránega, Amelia Eva2025-01-162025-01-162015-06-08Lozano-Velasco E, Vallejo D, Esteban FJ, Doherty C, Hernández-Torres F, Franco D, Aránega AE. A Pitx2-MicroRNA Pathway Modulates Cell Proliferation in Myoblasts and Skeletal-Muscle Satellite Cells and Promotes Their Commitment to a Myogenic Cell Fate. Mol Cell Biol. 2015 Sep 1;35(17):2892-909. doi: 10.1128/MCB.00536-15. Epub 2015 Jun 8. PMID: 26055324; PMCID: PMC4525317.0270-7306doi: 10.1128/MCB.00536-15.https://www.tandfonline.com/doi/10.1128/MCB.00536-15?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmedhttps://hdl.handle.net/10953/4016The acquisition of a proliferating-cell status from a quiescent state as well as the shift between proliferation and differentiation are key developmental steps in skeletal-muscle stem cells (satellite cells) to provide proper muscle regeneration. However, how satellite cell proliferation is regulated is not fully understood. Here, we report that the c-isoform of the transcription factor Pitx2 increases cell proliferation in myoblasts by downregulating microRNA 15b (miR-15b), miR-23b, miR-106b, and miR-503. This Pitx2c-microRNA (miRNA) pathway also regulates cell proliferation in early-activated satellite cells, enhancing Myf5  satellite cells and thereby promoting their commitment to a myogenic cell fate. This study reveals unknown functions of several miRNAs in myoblast and satellite cell behavior and thus may have future applications in regenerative medicine.CC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess