Gómez-Cruz , IreneContreras Gámez , María del MarRomero, InmaculadaRibeiro, BelinaRoseiro, Luisa B.Duarte, Luis C.Carvalheiro, FlorbelaCastro , Eulogio2025-02-122025-02-122023-11-15GÓMEZ-CRUZ, I., Contreras, M.d.M., Romero, I., Ribeiro, B., Roseiro, L.B., Duarte, L.C., Carvalheiro, F., Castro, E., 2023. Strategies for the purification of hydroxytyrosol-rich extracts obtained from exhausted olive pomace. Separation and Purification Technology, 325, 124664. https://doi.org/10.1016/j.seppur.2023.124664.1383-586610.1016/j.seppur.2023.124664https://doi.org/10.1016/j.seppur.2023.124664https://hdl.handle.net/10953/4669Exhausted olive pomace (EOP) is a residual biomass from which hydroxytyrosol can be recovered. This compound has applications in the food/pharma sectors, but its extraction yields complex extracts that require further purification for some applications. This work explores purification strategies based on membrane technology, liquid–liquid extraction (LLE), and solid-phase extraction with adsorbents and resins. The hydroxytyrosol content, phenolic profile, antioxidant activity, and inhibition of α-glucosidase and α-amylase were monitored. Hydroxytyrosol stood out in all purified extracts. The best hydroxytyrosol recovery (88.8%) was achieved using LLE with ethyl acetate as the extractant, while the purest extracts in phenolic compounds, including hydroxytyrosol, were obtained using the latter solvent and C18 (529 mg/g), DSC-8 (873 mg/g), and Purosorb PAD910 (523 mg/g). Conversely, mannitol and glucose, at high concentrations in the extract, were selectively retained in the aqueous phases. The developed strategies are discussed regarding their suitability to provide hydroxytyrosol-concentrated extracts, up to 291 mg/g, with antioxidant and antidiabetic functionalitiesengAttribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/TECHNOLOGY::Chemical engineeringinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess