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Titre: Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes
Auteur(s): Perán, Macarena
Marchal, Juan Antonio
López Ruiz, Elena
Jiménez Navarro, Manuel
Boulaiz, Houria
Rodríguez Serrano, Fernando
Carrillo, Esmeralda
Sánchez Espín, Gema
De Teresa, Eduardo
Tosh, David
Aránega, Antonia
Résumé: Background aims. The goal was to induce the transdifferentiation (or conversion) of human adipose-derived stem cells to cardiomyocytes using an intracellular extract obtained from adult human heart tissue. Methods. Human adult stem cells from lipoaspirates were transiently permeabilized, exposed to human atrial extracts and allowed to recover in culture. Results. After 21 days, the cells acquired a cardiomyocyte phenotype, as demonstrated by morphologic changes (appearance of binucleate, striated cells and branching fi bers), immunofl uorescence detection of cardiac-specifi c markers (connexin-43, sarcomeric a-actinin, cardiac troponin I and T, and desmin) and the presence of cardiomyocyte-related genes analyzed by reverse transcription – polymerase chain reaction (cardiac myosin light chain 1, a -cardiac actin, cardiac troponin T and cardiac b -myosin). Conclusions. We have demonstrated for the fi rst time that adult cardiomyocytes obtained from human donors retain the capacity to induce cardiomyocyte differentiation of mesenchymal stromal cells. The use of autologous extracts for reprogramming adult stem cells may have potential therapeutic implications for treating heart disease.
Mots-clés: adipose stem cells , cardiomyogenic differentiation , cell extract , transdifferentiation
Date de publication: 2010
metadata.dc.description.sponsorship: This study was supported by the Consejería de Salud (Junta de Andalucía) through project numbers PI-0384/2008 and TCRM 0014/2006. We are grateful to Dr J. C. Prados and Dra N. Rodríguez-Losada for their help and comments on the manuscript.
Editeur: Elsevier
Référence bibliographique: Perán M, Marchal JA, López E, Jiménez-Navarro M, Boulaiz H, Rodríguez-Serrano F, Carrillo E, Sánchez-Espin G, de Teresa E, Tosh D, Aranega A. Human cardiac tissue induces transdifferentiation of adult stem cells towards cardiomyocytes. Cytotherapy. 2010 May;12(3):332-7.
Collection(s) :DCS-Artículos

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